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http://dx.doi.org/10.5352/JLS.2004.14.6.938

Population Genetic Structure of Japanese Anchovy (Engraulis japonicus) in Korean waters Based on Mitochondrial 12S Ribosomal RNA Gene Sequences  

Kim Jin Yeong (South Sea Fisheries Research Institute, NFRDI)
Cho Eun Seob (South Sea Fisheries Research Institute, NFRDI)
Kim Woo Jin (Biotechnology Research Center, NFRDI)
Publication Information
Journal of Life Science / v.14, no.6, 2004 , pp. 938-950 More about this Journal
Abstract
We used portions of mitochondrial 125 ribosomal RNA gene sequences (339 bp) to investigate the phylogenetic and population genetic characteristics of the Japanese anchovy, Engraulis japonicus, in Korean waters. A total of 35 mtDNA haplotypes were obtained from the samples collected in 3 locations (the southern area of the Yellow Sea, the western coast of Jejudo, and the eastern area of the South Sea) in Korean waters. One haplotype, AN8T103, obtained from the southern area of the Yellow Sea, was formed according to an independent phylogenetic individual in the PAUP analysis, which was separated from the others by a $0.2-4.1\%$ sequence divergence. This distinct haplotype appeared to be one that was carried by immigrants from another study area, but further study is necessary. Genetic divergence, except for AN8T103, was moderate to substantial $(0.2-3.8\%)$ and nucleotide diversity within populations was 0.015 for Yellow Sea, 0.013 for Jejudo, and 0.D15 for South Sea, respectively. The female gene flow was substantial or high (Nm=25.5-36.4), and the genetic distances between regions were not statistically significant $(P>0.01)$. These results indicated that the Japanese anchovy populations occurring in Korean waters were consisted of individuals randomly dispersed over geographic areas.
Keywords
Genetic distance; genetic diversity; Japanese anchovy; Korean waters;
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1 Schneider, S., D. Roessli and L. Exocofier. 2000. Arlequin: A software for Population Genetics Data Analysis, ver. 2.0, Genetics of Biometry Laboratory, Department of Anthropology, University of Geneva, Switzerland
2 Sebastio, P., P. Zanelli and T. M. Neri. 2001. Identification of anchovy (Engraulis encrasicholus L.) and sardine (Sardinella aurita) by polymerase chain reaction sequence of their mitochondrial cytochrom b gene, and restriction analysis of polymerase chain reaction produce semipreserves. J. Agric. Food Chem. 49, 1194-1199   DOI   ScienceOn
3 Spanakis, E., N. Tsimenides and E. Zouros. 1989. Genetic differences between populations of sardine, Sardina pilchardus, and anchovy, Engraulis encrasicolus, in the Aegean and Ionina seas. J. Fish Biol. 35, 417-437   DOI
4 Swofford, D. L. 2000. Phylogenetic Analysis Using Parsimony ($^*and$ other Methods). version 4.0. Sinauer Associates, Sunderland, Massachusetts
5 Thomson, J. D., D. G. Higgins and T. J. Gibson. 1994. Clustal W: improving the sensitivity of progressive multiple sequences alignment through sequence weighting, position specific fap penalties and weight matrix choice. Nucl. Acid. Res. 22, 4673-4680   DOI   ScienceOn
6 Tudela, S., J. L. Garcia-Marin and C. Pla. 1999. Genetic structure of the European anchovy, Engraulis encrasicolus 1., in the north-west Mediterranean. J. Exp. Mar. Ecol. 234, 95-109   DOI   ScienceOn
7 Zhu, D., B. G. M. Jamieson, A. Hugall and C. Moritz. 1994. Sequence evolution and phylogenetic signal in control region and cytochrome b sequences of rainbowfishes (Melanotaeniidae). Mol. Biol. Evol. 11, 672-683
8 Chiu, T. S., Y. J. Lee, S. W. Huang and H. T. Yu. 2002. Polymorphic microsatellite markers for stock identification in Japanese anchovy (Engraulis japonica). Mol. Ecol. Notes 2, 49-50   DOI   ScienceOn
9 Grant, W. S. 1985. Biochemical population genetics of the southern African anchovy, Engraulis capensis. J. Fish Bio. 27, 23-29   DOI
10 Hellberg, M. E., R. S. Burton, J. E. Neigel and S. R. Palumbi. 2002. Genetic assessment of connectivity among marine populations. Bull. Mar. Sci. 70, 273-290
11 Inoue, J. G., M. Miya, K. Tsukamoto and M. Nishida. 2001. Complete mitochondrial DNA sequence of the Japanese anchovy, Engraulis japonicus. Fish. Sci. 67, 828-835   DOI   ScienceOn
12 Kim, J. Y. 1983. Distribution of anchovy eggs and larvae off the western and southern coasts of Korea. J. Kor. Fish. Soc. 16, 401-409. (in Korean)
13 Kim, J. Y. and Y. M. Choi. 1988. Vertical distribution of anchovy, Engraulis japonica eggs and larvae. J. Kor. Fish. Soc. 21, 139-144. (in Korean)
14 Kimura, M.1980. A simple method for estimating evolutionary rate of base substitution through comparative studies of nucleotide sequences. J. Mol. Evol. 116, 111-120
15 Lee, S. W. 1996. An outline of oceanophysics. Jipmundang Press, Seoul. 225pp
16 Avise, J. C. 1994. Molecular Markers, Natural History and Evolution. New York, 511 pp
17 Magoulas, A., N. Tsimenides and E. Zouros. 1996. Mitochondria DNA phylogeny and the reconstruction of population history of a species: the case of European anchovy (Engraulis encrasicolus). Mol. Biol. Evol. 13, 178-190   DOI   ScienceOn
18 Palumbi, S. R. 1994. Genetic divergence, reproductive isolation, and marine speciation. Ann. Rev. Ecol. Syst. 25, 547-572   DOI   ScienceOn
19 Asahida, T., T. Kobayashi, K. Saitoh and I. Nakayama. 1996. Tissue preservation and total DNA extraction from fish stored at ambient temperature using buffers containing high concentration of urea. Fish. Sci. 62, 727-730   DOI   ScienceOn
20 Bembo, D. G., G. R. Carvalho, M. Snow, N. Cingolani and T. J. Pitcher. 1995. Stock discrimination among European anchovies, Engraulis encrasicolus, by means of PCR- amplified mitochondria DNA analysis. Fish. Bull. 75, 31-40
21 Borsa, P. 2002. Allozyme, mitochondria-DNA, and morphometric variability indicate cryptic species of anchovy (Engraulis encrasicolus). Biol. J. Linn. Soc. 75, 261-270
22 Bembo, D. G., G. R. Carvalho, N. Cingolani and T. J. Pitcher. 1996. Electrophoretic analysis of stock structure in Northern Mediterranean anchovies, Engraulis encrasicolus. ICES J. Mar. Sci. 53, 115-128   DOI   ScienceOn