Browse > Article
http://dx.doi.org/10.5352/JLS.2004.14.5.855

Callus induction and plant regeneration from in vitro cultured petiole of 3 Gerbera cultivars.  

정용모 (경남농업기술원 화훼육종연구소)
나애실 (경남농업기술원 화훼육종연구소)
고은경 (동아대학교 생명자원과학대학)
송혜정 (경남농업기술원 화훼육종연구소)
김정부 (경남농업기술원 화훼육종연구소)
권오창 (동아대학교 생명자원과학대학)
Publication Information
Journal of Life Science / v.14, no.5, 2004 , pp. 855-858 More about this Journal
Abstract
The experiment was conducted to investigate optimal condition for callus induction and plant regeneration for transformation system of gerbera. Callus induction was more effective in 'white day' then other two cultivar 'Songsongee' and 'Love Song' The optimized plant growth regulators concentration on callus induction, was MS basal medium with NAA 0.1 mg/L＋ TDZ 0.5 mg/L. The optimized plant growth regulators concentration on plant regeneration, which was used MS basal medium was IAA 1.0 mg/L ＋ BA 1.0 mg/L ＋ Zeatin 0.1 mg/L. The optimized petiole age for more effective plant regeneration was 32 days petiole after in vitro subculture and MS basal medium strength was 1/2 MS strength.
Keywords
Gerbera; callus induction; plant regeneration; in vitro cultured petiole;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Teresa Orlikowska, Elzbieta Nowak, Agnieszka Marasek & Danuta Kucharska, 1999 'Effects of growth regulators and incubation period on in vitro renegeration of adventitious shoots from gerbera petioles', Plant Cell, Tissue and Organ Culture, 59, 95-102   DOI   ScienceOn
2 Tosca, A., L. Arcara and P. Frangi. 1999. Effect of genotype and season on gynogensis efficiency in gerbera. Plant Cell, Tissue and Organ Culture. 59, 77-80   DOI   ScienceOn
3 Orlikowska, T., E. Nowak, A. Marasek and D. Kucharska. 1999. Effects of growth regulators and incubation period on in vitro regeneration of adventitious shoots from gerbera petioles. Plant Cell Tissue and Org. Cul. 59, 95-102   DOI   ScienceOn
4 Pierik, R.L.M., H.H.M. Steegmans, and J.J. Marelis. 1973. Gerbera plantlets from in vitro cultivated capitulum explants. Scientia Hort. Sci. 28. 9-17(1973)
5 Pierik, R. L. M., J. L. M. Jansen, A. Maasdam and A. C. M.Binnendijk. 1975. Optimalization of Gerbera plantlet production from excised capitulum explants. Sci. Hortic. 3, 351-357   DOI   ScienceOn
6 Reynoird, J. P., D. Chrique, M. Noin, S. Brown and D. Marie. 1993. Plant regeneration from in vitro leaf culture of several Gerbera species. Plant Cell Tissue and Organ Culture. 33, 203-210   DOI   ScienceOn
7 Laliberte, S., L. Chretien, and J. Vieth. 1985. In vitro plantlet production from young capitulum explants of Gerbera jamesonii. Hortscience. 20(1), 137-139
8 Robinson, K. E. P. and E. Firoozababy. 1993. Transformation of floriculture crops. Scientia Hort. 55, 83-99   DOI   ScienceOn
9 Ruffoni, B. and F.Massabo. 1991. 'Tissue culture in Gerbera jamesonii hybrida', Acta Horticulturae, Plant Biotechonology, 289
10 Sitbon, M. 1981. Production of haploid Gerbera jamesonii plants by in vitro culture of unfertilised ovules. Agronomie. 1, 807-812   DOI
11 Miyoshi, K. and N. Asakura. 1996. Callus induction, regeneration of haploid plants and chromosome doubling in ovule cultures of pot gerbera (Gerbera jamesonii). Plant Cell Rep. 16, 1-5   DOI   ScienceOn
12 Elomaa P. , J. Honkanen, R. Puska, P. Suppanea, Y. Herariutta, M. Mehto, M. Kotilainen, L. Nevalainen and T. H. Teeri. 1993. Agrobacterium-mediated transfer of antisense chalcone synthase cDNA to Gerbera hybrida inhibits flower pigmentation. Bio/Tech. 11, 508-511   DOI   ScienceOn
13 Murashige T.,Serpa M., and B. Jones J. 1974. Clonal multiplication of gerbera through tissue culture. Hortscience, Vol. 9(3), 175-180
14 Nagaraju, V., G. S. L. Srinivas and G. Lakshmi Sita. 1998. Agrobacterium-mediated genetic transformation in Gerbera hybrida. Current Sci. 74(7), 630-634
15 Orlikowska, T. and E. Nowak.1997. Factors affecting transformation of Gerbera. Acta Hort. 447, 619-621
16 Grifing, B. 1959. Concept for general and specific combining ability in relation to diallel crossing system. Aust. J. Biological Sci. 9, 462-493
17 Jung, S. Y. 2000. Protoplasts Isolation and Culture System between Gerbera Hybrida Hort. 'Beauty' and Aster magnus. MS Thesis, Dong-A Univ
18 Hutchinson, J. F., V. Kaul, G. Maheswaran, J. R. Moran, M. W. Graham and D. Richards. 1992. Genetic improvement of floricultural crops using biotechnology. Aus. J. Bot. 40, 765-787   DOI
19 Jerzy, M. and M. Lubomski. 1992. In vitro adventitious bud techniques for mutation breeding of Gerbera jamesonii. Acta Hort. 314, 269-274
20 John M. Dole, Harold F. Wilkins. 1999. Florticulture Principles and species. Prenntice Hall. 356-361
21 Chung, Y. M., H. A. Kim, K. Y. Kim, S. W. Park, Y. B. Yi, J. H. Lee, and O. C. Kwon. 2001. Morphological characteristics and genetic variation of gerbera (Gerbera hybrida Hort.). Kor. J. Plant Biotech. 3(3), 113-121
22 Agriculture and Horticulture. 1997. Agriculture and Horticulture company. 125-135