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http://dx.doi.org/10.5352/JLS.2004.14.5.815

Antitumor activity of Bacillus subtilis SW-1 isolated from Jeotgal  

박종기 (진주산업대학교 미생물공학과)
조용운 (진주산업대학교 미생물공학과)
최영우 (신라대학교 식품영양학과)
정영기 (동의대학교 미생물학과)
갈상완 (진주산업대학교 미생물공학과)
Publication Information
Journal of Life Science / v.14, no.5, 2004 , pp. 815-820 More about this Journal
Abstract
A bacterum containing antitumor activity was isolated from traditional korean food, Jeotgal. Through the 16s rRNA sequence analysis, the bacterium was identitied as a strain of Bacillus subtilis SW-l. The best culture condition for antitumor activity of the bacterium is 3% of soluble starch and 1 % of yeast extract as corbon and nitrogen sources, respectively. Cytotoxicitic concentrations of the culture supernatant of B. subtilis SW-1 against cancer cell lines, A549 and SK-OV3 were 30 ul/ml and 40 ul/ml, respectively, as $IC_{50}$/ values. In DNA fragmentation assay, the culture supernatant showed the programmed cell death (apoptosis) to cause degrading the chromosomal DNA like ladder. Taken together, the culture supernatant of the B. subtilis SW-1 has some possibility to be used as an antitumor agent.
Keywords
Jeotgal; Bacillus subtilis; antitumor; apoptosis;
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