Browse > Article
http://dx.doi.org/10.5352/JLS.2004.14.5.794

Optimization of Cyclodextrin Glucanotransferase Immobilization on Amberlite IRA-900  

Seo, Hyo-Jin (Dept. of Biotechnology and Bioengineering, Pukyong National University)
Jung, Il-Hyong (Dept. of Biotechnology and Bioengineering, Pukyong National University)
Nam, Soo-Wan (Dept. of Biotechnology and Bioengineering, Pukyong National University)
Kim, Byung-Woo (Dept. of Biotechnology and Bioengineering, Pukyong National University)
Kim, Sung-Koo (Dept. of Biotechnology and Bioengineering, Pukyong National University)
Publication Information
Journal of Life Science / v.14, no.5, 2004 , pp. 794-799 More about this Journal
Abstract
Cyclodextrin glucanotransferase (CGTase) produced by Bacillus subtilis NAl/pKBl was used for the production of cyclodextrin (CD). The enzyme was purified by ion exchange and gel filtration chromatography. The purified enzyme exhibited its maximum activity in the pH range of 6.0 to 7.0 and temperature range of 60 to $70^{\circ}C$. Immobilization of purified CGTase was carried out with various immobilization matrices. Amberlite IRA-900, a strong basic anion exchange resin, showed the highest immobilization ability (38 units per gram resin). Optimal pH and temperature for enzymatic reaction of the immobilized CGTase were pH 6.0 and 60t. The activity of immobilized CGTase maintained more than a month and could be reused for a month in a continuous enzyme reactor for the production of CD.
Keywords
CGTase; Immobilization; Amberlite IRA-900; Cyclodextrin; Bacillus subtilis;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 Siana, H. K., Saidb, M., Hassanb, O., Kamaruddinc, K., Ismaila, A. F., Rahmana, R. A. Mahmooda, N. A. N., and Illiasa, R. 2004. Purification and characterization of cyclodextrin glucanotransferase from alkalophilic Bacillus sp. G1. Process Biochemistry, in press   DOI   ScienceOn
2 Sung, K. H., Kim, S. K., Jang, K. L. and Jeon, H. K. 2003. Immobilization of cyclodextrin glucanotransferase for production of 2-O-$\alpha$-D-glucopyranosyl L-ascorbic acid. Kor. J. Microbiol. Biotechnol. 31(4), 368-376   과학기술학회마을
3 Qi, Q., She, X., Endo T. and Immermann, W. 2004. Effect of the reaction temperature on the transglycosylation reactions catalyzed by the cyclodextrin glucanotransferase from Bacillus macerans for the synthesis of large-ring cyclodextrins Tetrahedron, 60(3), 799-806   DOI   ScienceOn
4 Tanaka, M., Muto, N. and Itaru, Y. 1991. Characterization of Bacillus stearothermophilus cyclodextrin glucanotransferase in ascorbic 2-O-$\alpha$-glucoside formation. Biochim. Biophys. Acta. 1078, 127-132   DOI   ScienceOn
5 Tonkova, A. 1998. Bacterial cyclodextrin glucanotransferase. Enz. Microbiol. Technol. 22, 678-686   DOI   ScienceOn
6 Kim, J. H., Hong, S. S. and Lee, H. S. 2001. Recovery of cyclodextrin glucanotransferase by adsorption to starch. Kor. J. Biotechnol. Bioeng. 16, 128-132   과학기술학회마을
7 Kim, P. S., Shin, H. D., Park, J. K. and Lee, Y. H. 2000. Immobilization of cyclodextrin glucanotransferase on Amberlite IRA-900 for biosynthesis of transglycosylated xylitol. Biotechnol. Bioprocess Eng. 5, 174-180   DOI   ScienceOn
8 Lee, M. S., Shin, H. D., Kim, T. K. and Lee, Y. H. 2004. Purification of α-cyclodextrin glucanotransferase excreted from thermophilic Geobacillus thermosacchalytycus and characterization of transglycosylation reaction of glycoside. Kor. J. Microbiol. Biotechnol. 32(1), 29-36
9 Lee, Y. H., Lee, S. H. and Shin, H. D. 1991. Performance of column type bioreactor packed with immobilized cyclodextrin glucacnotransferase for cyclodextrin production. J. Microbiol. Biotechnol. 1(1), 63-69
10 Mohamed, A. A., Reyad, M. R., Ahmed, F. A. 2000. Biosynthesis of cyclodextrin glucanotransferase by immobilized Bacillus amyloliquefaciens in batch and continuous cultures. Biochem. Eng. J. 5, 1-9   DOI   ScienceOn
11 Park, C. S., Woo, E. J., Kuk, S. U., Seo, B. C., Park, K. H. and Lim., H. 1992. Purification and characterization of cyclodextrin glucanotransferase from Bacillus sp. E1. Kor. J. Microbiol. Biotechnol. 20, 156-163
12 Park, D. C., Kim, T. K. and Lee, Y. H. 1998. Characteristics of transglycosylation reaction of cyclodextrin glucanotransferase in the heterogeneous enzyme reaction system using extrusion starch as a glucosyl donor. Enz. Microbiol. Technol. 22, 217-222   DOI   ScienceOn
13 Jeon, S. J., Nam, S. W., Yun, J. W., Song, S. K. and Kim, B. W. 1998. Effect of C- or D- domain deletion on enzymatic properties of cyclodextrin glucanotransfer- ase from Bacillus stearothermophilus NO2. J. Microbiol. Biotechnol. 8(2), 152-157
14 Fujiwara, S., H. Kakihara, B. W. Kim, A. Leujeune, M. Kanemoto, K. Sakaguchi, and T. Imanaka. 1992. Cyclization characteristics of cyclodextrin glucanotransferase are conferred by the $NH_2$-terminal region of the enzyme. Appl. Environ. Microbiol. 58, 4016-4025
15 Gawande, B. N., R. K. Singh, A. K. Chauhan, A. Goel, and A. Y. Patkar. 1998. Optimization of cyclomaltodextrin glucanotransferase production from Bacillus firmus. Enz. Microbiol. Technol. 22, 288-291   DOI   ScienceOn
16 In, M. J., Kim, D. C., Chae, H. J., Choi, K. S. and Kim, M. H. 1997. Immobilization of cyclodextrin glucanotransferase and its reaction characterisitics regarding transglucosylated stevioside production. Kor. J. Appl. Microbiol. Biotechnol. 25, 305-310
17 Kang, H. J., Chae, K. S. and Sunwoo, Y. I. 1995. Production of cyclodextrin glucanotransferase from alkalophilic Bacillus sp. C-21. Kor. J. Food Nutr. 8, 253-261   과학기술학회마을