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http://dx.doi.org/10.5352/JLS.2004.14.3.467

Isolation of Antimicrobial Substance by Produced Bacillus sp. SD-10 with Antagonistic Activity Towards Mushroom Pathogens  

이상원 (진주산업대학교 미생물공학과)
류현순 (진주산업대학교 미생물공학과)
갈상완 (진주산업대학교 미생물공학과)
박기훈 (경상대학교 농업생명과학과)
김철호 (진주산업대학교 미생물공학과)
최영주 (신라대학교 식품영양학과)
Publication Information
Journal of Life Science / v.14, no.3, 2004 , pp. 467-471 More about this Journal
Abstract
Bacillus sp. SD-10 was investigated to develope biological pesticides for control of mushroom diseases. Bacillus sp. SD-10 showed high antifungal activity when cultured at 35∼4$0^{\circ}C$ for 30∼4$0^{\circ}C$. The culture filtrate of the bacterium inhibited the growth of mycelium of T. virens which is a kind of mushroom pathogene. On the test of inhibition of spore germination of T. virens, more than 5% of the culture filtrate in the media inhibited completely the germination of the spores. An antimicrobial substance, UPX-1 was purified from the culture filtrate of the Bacillus. From the $^1H$-NMR and $^{13}C$-NMR spectrum analysis, the substance was indentifed as disaccharide composed to six carbon sugars. UPX-1 has not only strong antifungal activity against T. virens but also antibacterial activity against Pseudomonas tolaassi.
Keywords
antifungal activity; antibacterial activity; mushroom pathogene;
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