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http://dx.doi.org/10.5352/JLS.2004.14.3.429

Expression and Secretion of Zymomonas mobilis Levansucrase in Saccharomyces cerevisiae.  

임채권 (동의대학교 미생물학과)
김이경 (동의대학교 미생물학과)
김광현 (동의대학교 미생물학과)
김철호 (한국생명공학연구원)
이상기 (한국생명공학연구원)
남수완 (동의대학교 생명공학과)
Publication Information
Journal of Life Science / v.14, no.3, 2004 , pp. 429-434 More about this Journal
Abstract
Levansucrase gene (levU) from Zymomonas mobilis was subcloned downstream of GALl promoter in pYES 2.0 and pYInu-AT [GALl0 promoter＋exoinulinase signal sequence of Kluyveromyces marxianus], resulting pYES-levU and pYInu-levU, respectively. The two expression plasmids were introduced into an invertase-deficient strain, Saccharomyces cerevisiae SEY2102, and then transformants showing high activity of levansucrase were selected. When each yeast transformants was cultivated in medium containing galactose, the extracellular and intracellular activities of levansucrase reached about 7.17 U/㎖ with the strain harboring pYES-levU and 6.61 U/㎖ with the strain harboring pYInu-levU. It was found that about 50% of levansucrase were detected in the medium and periplasmic space, and exoinulinase signal sequence didn't enhance the secretion efficiency. Furthermore, the recombinant levansucrase expressed in yeast seems to be produced as a hyper-glycosylated form.
Keywords
Zymomonas mobilis; levansucrase; Saccharomyces cerevisiae; exoinulinase signal;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
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