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http://dx.doi.org/10.5352/JLS.2004.14.1.032

Phylogenetic Relationships Using ITS2 Sequence and RAPD-PCR Data from Four Species of Korean Pseudo-nitzschia (Bacillariophyceae)  

Cho, Eun-Seob (South Sea Fisheries Research Institute, Coastal Environment Management, MFRDI)
Lee, Young-Sik (South Sea Fisheries Research Institute, Coastal Environment Management, MFRDI)
Publication Information
Journal of Life Science / v.14, no.1, 2004 , pp. 32-37 More about this Journal
Abstract
A portion of ribosomal internal transcribed spacer (ITS) 2 was sequenced from the samples of Pseudo nitzschia (P. deticatissima, P. multiseries, P. pungens and P. subfraudulenta) to investigate the genetic characteristics by measuring tile magnitude of genetic diversity and the degree of similarity coefficient using random amplified polymorphic DNAs (RAPD)-PCR patterns. The phylogenetic trees inferred from the genetic distance analyses showed the placement of P. delicatissima formed a quite long distance from p. P. multiseries, P. pungens, and even P. subfraudulenta. The phylogenetic tree from RAPD patterns showed that P. multiseries and P. pungens had dissimilarity coefficient of 0.31, while P. delicatissima and three species of Pseudo-nitzschia had that of 0.81. It is likely thought that the genetic position of P. delicatissima formed far from P. multiseries, P. punges, and P. subfraudulenta. These results imply that ITS2 region is expected to support a useful molecular characters for recognizing at the species level and for even discriminating P. multiseries from P. pungens. RAPD method also will be used to differentiate the species of Pseudo-nitzschia in a short time.
Keywords
gene sequence; ITS2; phylogenetic analysis; Pseudo-nitzschia;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
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