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http://dx.doi.org/10.5352/JLS.2004.14.1.017

Effects of Monovalent Cations on the βReaction Kinetics of Tryptophan Synthase  

Kim, Il (Department of Molecular Biology, College of Natural Sciences, Pusan National University)
Shin, Hye-Ja (Environmental Engineering Major, Division of Applied Engineering, Dongseo University)
Im, Woon-Ki (Department of Molecular Biology, College of Natural Sciences, Pusan National University)
Kim, Han-Do (Department of Molecular Biology, College of Natural Sciences, Pusan National University)
Publication Information
Journal of Life Science / v.14, no.1, 2004 , pp. 17-20 More about this Journal
Abstract
Effects of monovalent cations were examined on the fast $\beta$reaction of $\alpha$D56N and $\alpha$D56G mutant tryptophan synthase. Reaction rates for the production and degradation of intermediates in the reaction were changed in the presence of cathons. The mutant proteins showed different reaction rates from those of wild-type protein, and additional changes occurred in the presence of cations. The results showed that monovalent cations and $\alpha$D56 are important in allosteric properties of this protein.
Keywords
allosterism; mutant protein; cation;
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