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http://dx.doi.org/10.5352/JLS.2003.13.5.699

Enhanced Discrimination of Listeria spp. Using RAPD Fingerprinting Complemented by Ribotyping-PCR  

임형근 (부산대학교 약학과)
홍종해 (강원대학교 수의학과)
박경진 (한국보건산업진흥원 HACCP팀)
최원상 (동국대학교 생명공학과)
Publication Information
Journal of Life Science / v.13, no.5, 2003 , pp. 699-704 More about this Journal
Abstract
The results typed by random amplification of polymorphic DNA (RAPD) were compared with those obtained by Enterobacterial repititive intergenic consensus (ERIC) fingerprinting and ribotyping-PCR. The discriminatory power of RAPD typing was the best among the methods tested. RAPD typing with two different primers for 13 Listeria spp. reference strains produced 11 patterns each. In contrast, ERIC fingerprinting produced 9 patterns and ribotyping-PCR produced 7 patterns each. Composite of two separate RAPD (Lis 11 and primer 6) results or RAPD (Lis11)/ ribotyping-PCR differentiated all 13 Listeria spp. reference strains. Therefore, composite of 2 separate RAPD (Lis11 and primer 6) or composite of RAPD (Lis11)/ribotyping-PCR is expected the most promising approach for typing field isolated Listeria spp. strains.
Keywords
random amplification of polymorphic DNA (RAPD); Enterobacterial repetitive intergenic consensus(ERIC) fingerprinting; ribotyping-PCR;
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Times Cited By KSCI : 1  (Citation Analysis)
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