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http://dx.doi.org/10.5352/JLS.2003.13.4.516

Isolation of protoplast from the marine red alga Porphyra pseudolinearis in Korea  

Kim, Young-Dae (Aquaculture Division, East Sea Regional Fisheries Research Institute)
Choi, Jae-Seok (Department of Biotechnology, Pukyong National University)
Lee, Ju (Aquaculture Division, East Sea Regional Fisheries Research Institute)
Son, Yong-Soo (Aquaculture Division, East Sea Regional Fisheries Research Institute)
Hong, Yong-Ki (Department of Biotechnology, Pukyong National University)
Publication Information
Journal of Life Science / v.13, no.4, 2003 , pp. 516-521 More about this Journal
Abstract
Optimal conditions of protoplast isolation from the seaweed Prophyra pseudolinearis have been described. The P. pseudolinearis is one of indigenous and dominant Porphyra species in the East Sea region. Protoplasts have been released by enzymatic treatment of 2% agarase and 2% hemicellulase in 25mM MES buffer, pH 6.0 containing 0.5 M sorbitol. The protoplasts could be fused with neutral red-stained protoplasts of P. okamurae by the addition of polyethylene glycol 8000 solution.
Keywords
protoplast; Prophyra pseudolinearis; cell fusion; osmotic stabilizers; enzyme solutions;
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