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http://dx.doi.org/10.5352/JLS.2003.13.2.154

Apoptotic Cell Death of Human Lung Carcinoma A549 Cells by an Aqueous Extract from the Roots of Platycodon grandiflorum  

이성열 (동의대학교 한의과대학 내과학교실)
이재훈 (동의대학교 한의과대학 생화학교실 및 한의학연구소)
김원일 (동의대학교 한의과대학 내과학교실)
배송자 (신라대학교 자연과학대학 식품영양학과)
박동일 (동의대학교 한의과대학 내과학교실)
최영현 (동의대학교 한의과대학 생화학교실 및 한의학연구소)
Publication Information
Journal of Life Science / v.13, no.2, 2003 , pp. 154-162 More about this Journal
Abstract
Platycodi Radix, the root of Platycodon grandiflorum, commonly known as Doraji, is used as a traditional oriental medicine. Extracts from the roots of P grandiflorum have been reported to have wide ranging health benefits. In the present study, we investigated the effects of an aqueous extract from the roots of P. grandiflorum (AEPG) on the growth of human lung carcinoma A549 cells. Upon treatment with AEPG, a concentration-dependent inhibition of cell growth was observed and cells developed many of the hallmark features of apoptosis, including condensation of chromatin. Flow cytometry analysis confirmed that AEPG increased populations of apoptotic-sub Gl phase. Immunoblot and quantitative RT-PCR analyses indicated that the expressions of Bcl-2 was down-regulated but Bax was up-regulated in AEPG-treated A549 cells. The expression of active form of caspase-3 by AEPG treatment was markedly increased, and the levels of poly(ADP-ribose) polymerase (PARP) and $\beta$-catenin, its target proteins, were decreased in a concentration dependent manner. Taken together, these findings suggest that P. grandiflorum has strong potential for development as an agent for prevention against human lung cancer.
Keywords
Platycodon grandiflorum; Lung cancer cells; Apoptosis; Bcl-2; Bax; Caspase-3;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
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