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http://dx.doi.org/10.5352/JLS.2003.13.1.105

Development of Recombinant Saccharomyces cerevisiae Expressing Epoxide Hydrolase for the Preparation of Chiral Phenyl Oxirane  

이수정 (경성대학교 공과대학 식품공학과)
이은정 (경성대학교 공과대학 식품공학과)
김초희 (경성대학교 공과대학 식품공학과)
이지원 (경성대학교 공과대학 식품공학과)
김희숙 (경성대학교 공과대학 식품공학과)
이은열 (경성대학교 공과대학 식품공학과)
Publication Information
Journal of Life Science / v.13, no.1, 2003 , pp. 105-109 More about this Journal
Abstract
Epoxide hydrolase (EH) gene from Aspergillus niger #33 was cloned from cDNA library generated by reverse transcriptase-polymerase chain reaction (RT-PCR). The nucleotide sequence analysis revealed that the deduced amino acid sequence was almost similiar to that of A. niger LCP521 previously reported. The cloned EH gene was transformed into Saccharomyces cerevisiae and expressed by addition of galactose. The recombinant S. cerevisiae showed hydrolytic activity toward racemic phenyl oxirane substrate based on chiral GC analysis, and can be used as a potential biocatalyst for the preparation of chiral phenyl oxirane.
Keywords
Aspergillus niger; epoxide hydrolase; cloning; chiral Phenyl oxirane;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
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