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http://dx.doi.org/10.5352/JLS.2003.13.1.001

Antioxidant Activity and Biological Properties in Extracts of Euonymus alatus (Thnub.) Sieb.  

서경수 (안동대학교 자연과학대학 생명자원과학부)
임종국 (안동대학교 자연과학대학 생명자원과학부)
박재호 (안동대학교 자연과학대학 생명자원과학부)
김충현 (안동대학교 자연과학대학 생명자원과학부)
정규영 (안동대학교 자연과학대학 생명자원과학부)
정형진 (안동대학교 자연과학대학 생명자원과학부)
Publication Information
Journal of Life Science / v.13, no.1, 2003 , pp. 1-8 More about this Journal
Abstract
Antioxidant activity and biological properties in the MeOH extracts from different plant parts of Euonymus alatus (THNUB.) were measured by DPPH free radical scavenging ability and inhibition ability against xanthine/xanthine oxidase and proliferation in HL-60 cells. DPPH free radical scavenging activities in extracts of plant parts were high such as leaf, wing, root, seed and stem, respectively. The EtOAc fractions of plant parts were purified through LH-20 column chromatography and identified by GC/MS. LH-4 fraction and LH-5 fraction of leaf, stem and root showed stronger activities than other fractions in the inhibitor activity of DPPH and xanthine/ xanthine oxidase. $IC_{50}$ values of LH-4 fraction eluted from stem extracts showed such as 2.38 and 5.32 in DPPH and xanthine/ xanthine oxidase assay. Polyphenolic compounds were identified in purified LH-20 fractions showed highest $IC_{50}$ value in DPPH and xanthine/xanthine oxidase assay The activity of POD according to sampling time was high in root harvested in May and leaf harvested in September, respectively. The activity of SOD showed only in the extracts of stem in plant parts. SOD and POD in leaf were similar in the patterns of isozyme to those of stem. The purified extracts from Euonymus alatus (THNUB.) exerted inhibition ability of proliferation in HL-60 cells.
Keywords
DPPH; xanthine oxidase; Euonymus alantus (Thnub.) Sieb; SOD; POD; HL-60 ell;
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Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Epidemiological evidence on potential health properties of flavonoids /
[ Hertog, M. G. ] / Proc. Nutr. Soc.   DOI   ScienceOn
2 In vivo antioxidant effect of green and black tea in man /
[ Serafini, M.;A. Ghiselli;A. Ferro-Luzz ] / Eur. Clin. Nutr.
3 Biological effects of the superoxide radical /
[ Fridovich, I. ] / Arch. Biochem. Biophys.   DOI   ScienceOn
4 Polyphenols as cancer chemopreventive agents /
[ Stoner, G. D.;H. Mykhtar ] / J. Cell. Bio. Chem.
5 Inhibitory effect of curcumin, Chlorogenetic acid, caffeic acid and Ferulic acid on tumor promotion in mouse skin by 12-o-tetradecanoylphorbol 13-acetate /
[ Huang, M. T.;R. C. Smart;C. Q. Wong;A. H. Conney ] / Cancer Res.
6 /
[ Alscher, R. G;J. L. Hess ] / Antioxidants in higher plants
7 Vitamin nature of flavones /
[ Benthsath, A.;S. Rusznyak;A. Szent-Gyorgyi ] / Nature   DOI
8 Consumption of black tea and cancer risk : a prospective cohort study /
[ Goldbohm,R. A.;M. G. Hertog;H. A. Brant;G. Van Poppel;P. A. Van der Brandt ] / J. Natl. Cancer Inst.   DOI
9 Evaluation of cancer chemo-preventive activity mediated by antioxidants and modulators of tumor promotion /
[ Lee. S. K. ] / Phd thesis of university of illinois at Chicago
10 Antioxidant properties of 2-imidazolones and 2-imidazolthiones /
[ Smith, R. C.;J. C. Reeves;R. C. Dage;R. A. Schnettler ] / Biochem. Pharmacol.   DOI   ScienceOn
11 Herbal remedies of the maritime indians /
[ Chandler, F. R.;L. Freeman;S. N. Hooper ] / J. Ethnopharmacol.   DOI   ScienceOn
12 Antiallergic constituents from oolong tea stem /
[ Ohmori, Y.;M. Ito;M. Kishi;H. Mizutani;T. Karada;H. Konishi ] / Biol. Pharm. Bull.   DOI   ScienceOn
13 The free radical theory of aging matures /
[ Beckman, K. B.;B. N. Ames ] / Physiol. Rev.
14 Inhibitory effects of phenolics on xanthine oxidase /
[ Chang,W. S.;Y. H. Chang, F. J. Lu;H. C. Chiang ] / Anticancer Res.
15 /
[ Tally, F. P.;H. R. Godin;N. V. Jacobus;S. L. Gorbach ] / Infact. Immun.
16 Aging: A thory based on free radical and radiation chemistry /
[ Harman, D. ] / J. Gerontol.   DOI   ScienceOn
17 Antioxidant properties of hydroxy-flavones /
[ Cotelle, N.;J. L. Bernier;J. P. Catteau;J. Pommery;J. C. Wallet;E. M. Gaydou ] / Free Radic. Biol. Med.   DOI   ScienceOn
18 Oxidative damage and mitochondrial decay in aging /
[ Shigenaga, M.;T. M. Hagen;B. N. Ames ] / Proc. Natl. Acad. Sci. USA.   DOI   ScienceOn
19 Toxicological studies of antioxidants butylated hydroxytoluene (BHT) and buthylated hydroxyanisole(BHA)(in Korean) /
[ Choe, S. Y.;K. H. Yang ] / Korean J. Food Sci, Technol.
20 Antitumor activity of crude sesaminol in sesame seed /
[ Ryu, S.N.;B. H. Lee ] / Korean J. Crop. Sci.   과학기술학회마을
21 Structure activity relationship of caffeic acid analogues on xanthine oxidase inhibition /
[ Chan, W. S.;P. C. Wen;H. C. Chiang ] / Anticancer Res.
22 Studies on antioxidative substance in sesame seed /
[ Fukuta, Y.;T. Osawa;M. Namiki;Ozaki, T. ] / Agric. Biol. Chem.   DOI
23 Antibacterial effect of theaflavin, polyphenon 60(Camellia Sinensis) and euphorbia hirta on shigella spp. /
[ Vijaya, K.;S. Ananthan;R. Nalini ] / J. Ethnopharmacol.   DOI
24 Superoxide dismutase. An enzymic function for erythrocuprein (Hemocuprein) /
[ McCord, J. M.;I. Fridovich ] / J. Biol. Chem.
25 /
[ Hassan, H. M.;Armstrong, D(ed.);R. S. Sohal(ed.);R. G. Cutler(ed.);T. F. Slater(ed.) ] / Free Radicals in Molecular Biology, Aging and Disease
26 Over-expression of copper/zinc superoxide dismutase in the cytosol of transgenic tobacco confers partial resistance to ozone-induced foliar nerosis /
[ Pitcher, L. H.;B. A. Zilinskas ] / Plant Physiol.
27 A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding /
[ Bradford, M. M. ] / Anal. Biochem.   DOI   ScienceOn
28 Inflammation and oxidative stress in carcinogenesis /
[ Cerutti, P. A.;B. F. Trump ] / Cencer cell
29 Studies on the inhibitory effects of qurcetin of the growth of HL-60 Leukemia cells /
[ Kang, T. B.;N. C. Liang ] / Biochem. Pharmacol.   DOI   ScienceOn
30 Superoxide dismutase : Improved assays and an assay applicable to acrylamide gels /
[ Beauchamp, C.;I. Freidovich ] / Anal Biochem.   DOI   ScienceOn
31 Dissection of oxidative stress tolerance using transgenic plants /
[ Allen, R. D. ] / Plant Physiol.
32 Superoxide and hydrogen peroxide in relation to mammalian cell proliferation /
[ Barry, R. H. ] / Free Radical Biol. & Medi.   DOI   ScienceOn
33 The effects of theanine, as a novel biochemical modulator, on the antitumor activity of adriamycin /
[ Sadzuka, Y.;A. Sugiyama;Y. Nozawa;S. Hirota ] / Cancer Lett.   DOI   ScienceOn