Browse > Article
http://dx.doi.org/10.5352/JLS.2002.12.6.682

Characterization of the enzymatic property of thermostable carboxypeptidase Taq by addition of metal ions and replacement of active center metal  

Lee, Sang-Hyeon (Department of Bioscience and Biotechnology, Silla University)
Ha, Jong-Myung (Department of Bioscience and Biotechnology, Silla University)
Ha, Bae-Jin (Department of Bioscience and Biotechnology, Silla University)
Publication Information
Journal of Life Science / v.12, no.6, 2002 , pp. 682-687 More about this Journal
Abstract
We analyzed improvement on the enzyme activity of CPase Taq by addition of various metal ions. The enzyme activity was increased more then four times by 1 mM cobalt ion and almost three times by 1 mM calcium ion. However, the active center metal zinc ion did not affect the enzyme activity. In order to investigate whether the active center metal affects the enzyme activity, zinc ion which is occupied the active center of the enzyme was replaced by cobalt ion which activates the enzyme activity very effectively. Since the cobalt ion in the active center of the cobalt-substituted CPase Taq did not affect the enzyme activity, it could act as the natal metal ion in the active center of the enzyme.
Keywords
carboxypeptidase; zinc-dependent; thermostable; cobalt-substituted;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Purification and characterization of a thermostable carboxypeptidase (carboxypeptidase Taq) from Thermus aquaticus YT-1 /
[ Lee, S.-H.;E. Minagawa;H. Taguch;H. Matsuzawa;T. Ohta;S. Kaminogawa;K. Yamauchi ] / Biosci. Biotech. Biochem.   DOI   ScienceOn
2 Carboxypeptidase A /
[ Harsuck, J. A.;W. N. Lipscomb;Boyer(ed.) ] / The enzymes: Hydrolysis peptide bonds(3rd ed.)   DOI
3 The active site of carboxypeptidase Taq possesses the active-site motif His-Glu-X-X-His of zinc-dependent endopeptidases and aminopeptidases /
[ Lee, S.-H.;H. Taguchi;E. Yoshimura;E. Minagawa;S. Kaminogawa;T. Ohta;H. Matsuzawa ] / Protein Engineer   DOI
4 Carboxypeptidase Taq, a thermostable zinc enzyme, from Thermus aquaticus YT-1: olecular cloning, sequencing, and expression of the encoding gene in Escherichia coli /
[ Lee, S.-H.;H. Taguchi;E. Yoshimura;E. Minagawa;S. Kaminogawa;T. Ohta;H. Matsuzawa ] / Biosci. Biotech. Biochem.   DOI   ScienceOn
5 Enkephalin convertase: purification and characterization of a specific enkephalin-synthesizing carboxypeptidase localized to adrenal chromaffin granules /
[ Fricker, L. D.;S. H. Snyder ] / Proc. Natl. Acad. Sci. USA   DOI   ScienceOn
6 Bohr-effect and pH-dependence of electron spin resonance spectra of a cobalt-substituted monomeric insect haemoglobin /
[ Gersonde, K.;H. Twilfer;M. Overkamp ] / Biophys. Struct. Mech.   DOI   ScienceOn
7 Structure of the active site of carbonic anhydrase as determined by electron spin resonance /
[ Haffner, P. H.;J. E. Coleman ] / J. Biol. Chem.
8 A modified ninhydrin colorimetric analysis for amino acids /
[ Rosen, H. ] / Arch. Biochem. Biophys.   DOI   ScienceOn
9 Primary structure of bovine carboxypeptidase B. Inferences from the locations of the half-cystines and identification of the active site arginine /
[ Schmidt, J. J.;C. H. Hirs ] / J. Biol. Chem.
10 Cobalt bovine superoxide dismutase. Reactivity of the cobalt chromophore in the copper-containing and in the copper-free enzyme /
[ Calabrese, L.;D. Cocco;L. Morpurgo;B. Mondovi;G. Rotilio ] / Eur. J. Biochem.   DOI   ScienceOn
11 Reconstitution of <TEX>$Cu^{2+}$</TEX> -depleted bovine serum amine oxidase with <TEX>$Co^{2+}$</TEX> /
[ Agostinelli, E.;G. De Matteis;B. Mondovi;L. Morpurgo ] / Biochem J.
12 Cleavage of structural proteins during the assembly of the head of bacteriophage T4 /
[ Laemmli, U. K. ] / Nature   DOI   ScienceOn
13 The amino acid sequence of bovine carboxypeptidase A /
[ Bradshaw, R. A.;L. H. Ericsson;K. A. Walsh;H. Neurath ] / Proc. Natl. Acad. Sci. USA   DOI   ScienceOn