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http://dx.doi.org/10.5352/JLS.2002.12.5.584

Development of Asymmetric Resolution System for the Production of Chiral Styrene Oxide by Microbial Epoxide Hydrolase  

이지원 (경성대학교 공과대학 식품공학과)
윤여준 (울산대학교 공과대학 화학공학과)
이은열 (경성대학교 공과대학 식품공학과)
Publication Information
Journal of Life Science / v.12, no.5, 2002 , pp. 584-588 More about this Journal
Abstract
Asymmetric enantioselective resolution system using epoxide hydrolase activity of Aspergillus niger LK was developed and operated for the production of optically pure styrene oxide. Two-phase hollow-fiber reactor system was employed for the enhanced solubility of racemic styrene oxide in organic phase and protection of epoxide hydrolase activity in aqueous phase. For the removal of phenyl-1,2-ethandiol, the inhibitor of epoxide hydrolase, cascade hollow-fiber reactor system was also developed. Chiral (S)-styrene oxide (39 mM in dodecane) could be asymmetrically resolved with high enantiopurity (> 99% ee) using these reactor system.
Keywords
asymmetric resolution; hollow-fiber reactor; chiral styrene oxide; Anpergillus niger LK;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
연도 인용수 순위
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