Browse > Article
http://dx.doi.org/10.5352/JLS.2002.12.3.325

Antioxidative activity of flavonoid rich extract of Oenothers odorate Jacquin on oxidation of low density lipoprotein  

Ryu, Beung-Ho (Department of Food Science and Biotechnology)
Kim, Hee-Sook (Department of Food Science and Biotechnology)
Cho, Kyung-Ja (Department of Home Economic)
Publication Information
Journal of Life Science / v.12, no.3, 2002 , pp. 325-331 More about this Journal
Abstract
There is growing interest ill understanding the role and mechanisms of flavonoid as antioxidant on LDL. The antioxidative activity of flavonoid typically present in Oenothers odorate Jacquin was investigated in vitro using a human LDL oxidation assay. In present work, LDL was incubated with increasing concentrations of extracts of extracts of Oenothers odorate Jacquin and LDL oxidation was started by adding CuSO$_4$to the media. Substances in leaves extracts of Oenothers odorate Jacquin are capable of inhibiting the initiation and the propagation of LDL oxidation. They inhibit LDL oxidation, monitored by thiobarbituric acid-reactive substances(TBARS), as well as modification as shown through direct measurement of electrophoretic mobility, diene conjugates. Inhibition is a dose dependent effect that becomes already apparent at concentration of extracts as low as 40$\mu\textrm{g}$/mL. Inhibition is almost complete at 80$\mu\textrm{g}$/mL. Extracts of Oenothers odorate Jacquin were more potent antioxidative activity than either ascorbic acid and dl-a -tocopherol.
Keywords
Low density lipoprotein(LDL); antioxidative activity; Oenothers odorate Jacquin; flavonoid;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Roma, P., A. L. Catapano, S. M. Bertulli, L. Varesi, R.Fumagalli and F. Bernini. 1990. Oxidized LDL increase free cholesterol and fail to stimulate cholesterol esterification in murine macrophages. Biochem. Biophys. Res. 171, 123-131   DOI   ScienceOn
2 Ryu, B. H., J. W. Jeung, L. G. Robert and P. Greenspan.1990. Antioxidative activitv for human low density lipoprotein oxidation by a novel compound purified form marine microbial origin. J. Marine Biotech. 81, 175-182
3 Steinbrecher, U., S. Parthasarathy, D. S. Leake, J. L Witztum and D. Steinberg. 1984. Modification of low density lipoprotein by endothelial cells involves lipid peroxidation and degradation of low density lipoprotein phospholipids. Proc. Natl. Acad. Sci. USA 83, 3883-3887
4 Cristol, L. S., I. Jialal and M. Grundy. 1992. Effect of low-dose protocol therapy on LDL oxidation and the plasma lipoprotein profile in male volunteers. Atherosderosis. 97, 10-20
5 Cominacini, L, U. Garbin, B. Cenci, A. Davoli, E. Ratti, G. Gaviraghi, V. Lo Cascio and A. M. Pastorino. 1991. Predisposition to LDL oxidation during copper-catalyzed oxidative modification and its relation to alpha-tocopherol content in Humans. Clin. Chim. Acta. 204, 57-68   DOI   PUBMED   ScienceOn
6 Esterbauer, H., G. Striegl, H. Puhl and M. Rotheneder.1989. Continuous monitoring of in vitro oxidation of human low density lipoprotein. free Radic. Res. Commun. 6, 67-75   DOI
7 Frankel, E. N., J. Kamer, J. B. German and J. E. Kinsella. 1993. Inhibition of oxidation human lowdensity lipoprotein by phenolic substances in red wine. Lancet. 341, 454-457   DOI   ScienceOn
8 Frei, B., Stocker, R. and B. N. Ames. 1977. Antioxidant defenses and lipid peroxidation in human blood plasma. Proc. Natl. Acad. Sci. USA 85, 9748-9752   DOI   ScienceOn
9 Jialal, I. and C. Scaccini. 1992. Antioxidants and atherosclerosis. Curr. Opin. Lipidol. 3, 324-328   DOI
10 Havel, R. J., H. A. Eder and J. H. Bragdon. 1995. The distribution and chemical composition of ultracentrifugally separated lipoproteins in human serum. J. CIin. Invest. 34, 1345-1352
11 Ross, R. 1993. The pathogenesis of atherosclerosis perspective for the 1990. Nature, 362, 801-809   DOI   PUBMED   ScienceOn
12 Lowry, O. H., N. J. Rosebrough, A. L. Farr and R. J. Randall. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193, 265-275
13 Tallahda, R. J. and R. B. Murray. 1987. Manual of pharmacological calculations with computer programs, 2nd (ed), Springer. Verlag. N. Y, pp. 145-148
14 Yaki, K. 1976. A simple fluorometric assay for lipoprotein in blood plasma. Biochem. Med. 15, 212-217   DOI   PUBMED   ScienceOn
15 Heinecke, J. W., H. Rosen and A. Chait. 1984. Iron and copper promote modification of low density lipoprotein by human arterial smooth muscle cells in culture. J. Clin. Invest. 74, 1890-1894   DOI   PUBMED
16 Bridges, A. B., N. A. Scoot and J. F. Belch. 1991. Protocol, a superoxide free radical scavenger in vitro. Atherosderosis. 89, 263-265   DOI   ScienceOn
17 Robak, J., F. Shridi, M. Wolbis, M. Krolikowska and J. Sol. 1988. Screening of the influence of flavonoids on lipoxygenase and cycloxygenase activity as well as on non-enzymatic lipid oxidation. Pharmacol. Pharm. 40, 451-458   DOI
18 Jiala, I., G. L. Vega and S. M. Grundy. 1990. Physiologic levels of ascorbate inhibit the oxidative modification of low density lipoproteins. Atherosclerosis 82, 185-191   DOI   ScienceOn
19 Greenspan, P. and R. L. Gutman. 1993. Detection by Nile red of agarose gel electrophoresised native and modified low density lipoprotein. Etectrophoresis. 14, 65-68   DOI   ScienceOn
20 Bonanome, A., A. Pagnan, S. Biffanti, A. Opportuno, F. Sorgato, M. Dorella, M. Maiorino and F. Ursini.1992. Effect of dietary mono unsaturated and poly-unsaturated fatty acids on the susceptibility of plasma low density lipoproteins to oxidative modification. Arteriosder. Thromb. 12, 529-533   DOI
21 Esterbauer, H., H. Puhl, M. Dieber-Rotheneder, G. Waeg and H. Rabl. 1991. Effect of antioxidants on oxidative modification of LDL. Ann. Med. 23, 573-581   DOI   ScienceOn
22 Henriksen, T., E. Mahoney and D. Steinberg. 1981. Enhanced macrophage degradation of low density lipoportein previously incubated with cultured endothelial cells: Recognition by the receptor for acetylated low density lipoprotein. Proc. Natl. Acad. Sci. USA 78, 6499-6503   DOI   ScienceOn
23 Renaud, D. and M. Lorgeril. 1993. Wine, alcohol, platelets and the French Paradox for coronary heart disease. Lancet. 239, 1523-1525
24 Morel, D. W., P. E. Dicorleto and G. M. Chisholm.1984. Endothelial and smooth muscle cells after low density lipoproteins in vitro by free radical oxidation. Arteriosderosis 4, 357-364   DOI
25 Esterbauer, H., H. Puhl, M. Dieber-Rotheneder, G.Striegl and G. Waeg. 1991. Role of vitamin E in preventing the oxidation of low density lipoprotein. Am. J. Clin. Nutr. 53, 314S-321S   PUBMED