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http://dx.doi.org/10.5352/JLS.2002.12.3.264

Cloning and Expression of Antifungal Protein (PR5) Genes from Hot Pepper (Capsicum annuum L.)  

Park, Hae-Jin (Department of Agronomy, College of Agriculture, Kyungpook National University)
Lee, Jung-Hoon (Department of Agronomy, College of Agriculture, Kyungpook National University)
Yoon, Yong-Hwi (Department of Agronomy, College of Agriculture, Kyungpook National University)
Kim, Hak-Yoon (Faculty of Environmental Studies, Keimyung University)
Shin, Dong-Hyun (Department of Agronomy, College of Agriculture, Kyungpook National University)
Lee, In-Jung (Department of Agronomy, College of Agriculture, Kyungpook National University)
Kim, Dal-Ung (Department of Agronomy, College of Agriculture, Kyungpook National University)
Kim, Kil-Ung (Department of Agronomy, College of Agriculture, Kyungpook National University)
Publication Information
Journal of Life Science / v.12, no.3, 2002 , pp. 264-273 More about this Journal
Abstract
We have isolated and artificially expressed three cDNA clones of Capsicum annuum PR5 genes for elucidating the antifungal activity against Phytophthora capsici which contracted a hot pepper root rot in field condition. Three divergent PR5 proteins from hot pepper were designated as CAPR5-1 and CAPR5-2 from susceptible cultivar (Subicho) as well as CAPR5-3 from resistant cultivar (CM331) in response to P. capsici. The cDNA similarity was found over 80% of identity among the three CAPR5s, and deduced amino acid sequence was characterized that all of CAPR5s contained 16 cysteine residues which possibly had a significant role in the structural formation. The result of genomic DNA blot showed that CAPR5-1 and CAPR5-2 existed as single copy in the Subicho genome. Three recombinant CPARs in E. coli were identified by SDS-PACE, and each expressed protein was treated on the PDA medium which contained cultured pathogens. Although three CAPR5 proteins did not affected the hyphal growth of Glomerella glycines and Colletotrichum fagenarium, CAPR5-1, CAPR5-2, and CAPR5-3 showed a specific antifungal activities against P. capsici.
Keywords
Hot pepper; Osmotic-like protein; Pathogenesis-related protein;
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