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http://dx.doi.org/10.5423/RPD.2021.27.4.180

Occurrence of Leaf Spot Disease on Watermelon Caused by Pseudomonas syringae pv. syringae  

Park, Kyoung-Soo (Nongwoo Bio Co., Ltd.)
Lee, Ji-Hye (Nongwoo Bio Co., Ltd.)
Kim, Young-Tak (Nongwoo Bio Co., Ltd.)
Kim, Hye-Seong (Nongwoo Bio Co., Ltd.)
Lee, June-woo (Nongwoo Bio Co., Ltd.)
Lee, Hyun-Su (Animal and Plant Quarantine Agency)
Lee, Hyok-In (Animal and Plant Quarantine Agency)
Cha, Jae-Soon (Department of Plant Medicine, Chungbuk National University)
Publication Information
Research in Plant Disease / v.27, no.4, 2021 , pp. 180-186 More about this Journal
Abstract
Typical bacterial symptoms, water-soaking brown and black leaf spots with yellow halo, were observed on watermelon seedlings in nursery and field of Gyeongnam and Jeonnam provinces. Bacterial isolates from the lesion showed strong pathogenicity on watermelon and zucchini. One of them was rod-shaped with 4 polar flagella by observation of transmission electron microscopy. They belonged to LOPAT group 1. The phylogenical trees with nucleotide sequences of 16S rRNA and multi-locus sequencing typing with the 4 house-keeping genes (gapA, gltA, gyrB, and rpoD) of the isolates showed they were highly homologous to Pseudomonas syringae pv. syringae and grouped together with them, indicating that they were appeared as P. syringae genomospecies group 1. Morphological, physiological, and genetical characteristics of the isolates suggested they are P. syringae pv. syringae. We believe this is the first report that P. syringae pv. syringae caused leaf spot disease on watermelon in the Republic of Korea.
Keywords
Bacterial leaf spot; New disease; Pseudomonas syringae pv. syringae; Watermelon;
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