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http://dx.doi.org/10.5423/RPD.2020.26.1.29

First Report of Charcoal Rot Caused by Macrophomina phaseolina on Glycine max in Korea  

Ko, Young Mi (Hwaseong city Agricultural Technology Center)
Choi, Jiyoung (Department of Plant Medicine, College of Agriculture, Life & Environment Sciences, Chungbuk National University)
Lee, Yeong Hee (Division of Horticultural Environment, NHRI)
Kim, Heung Tae (Department of Plant Medicine, College of Agriculture, Life & Environment Sciences, Chungbuk National University)
Publication Information
Research in Plant Disease / v.26, no.1, 2020 , pp. 29-37 More about this Journal
Abstract
Stem blight symptom of soybean was severely developed in 2016 in Hwaseong and Yeoncheon. During the seedling period, the damping-off of seedlings and the brown or black spots of cotyledons were observed. After August, the leaves began to be yellowed, and partially browned areas on leaves began to develop. After September, microsclerotia began to form even on the surface of the stems that had exhibited water-soaking symptom. After mid-October of the harvest season, the epidermis of the stem was peeled off, resulting in the formation of a large number of microsclerotia in the cortex. The pathogens isolated from these symptoms were the best in mycelial growth at 32-35℃, and the formation of microsclerotia was the most at 20-28℃. The pathogen was identified as Macrophomina phaseolina through the morphological characteristics of the pathogen and the sequencing of the internal transcribed spacer region gene. In addition, when inoculated with a soybean stem using toothpicks cultured with the pathogen, the same symptoms as seen on the soybean field occurred. When the pathogen was re-isolated at the lesion site, the same pathogen was isolated and identified as Macrophomina phaseolina. Based on the results, the disease is reported as soybean charcoal rot.
Keywords
Charcoal rot; Macrophomina phaseolina; Stem blight of soybean;
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1 Allen, T. W., Bradley, C. A., Sisson, A. J., Byamukama, E., Chilvers, M. I., Coker, C. M. et al. 2017. Soybean yield loss estimates due to diseases in the United States and Ontario, Canada, from 2010 to 2014. Plant Health Prog. 18: 19-27.   DOI
2 Chidambaram, P. and Mathur, S. B. 1975. Production of pycnidia by Macrophomina phaseolina. Trans. Br. Mycol. Soc. 64: 165-168.   DOI
3 Cummings, J. A. and Bergstrom, G. C. 2013. First report of charcoal rot caused by Macrophomina phaseolina in soybean in New York. Plant Dis. 97: 1506.   DOI
4 de los Santos, B., Aguado, A., Borrero, C., Viejobueno, J. and Aviles, M. 2019. First report of charcoal rot, caused by Macrophomina phaseolina, on blueberry in southwestern Spain. Plant Dis. 103: 2677.   DOI
5 Gopala, Gogoi, R., Hooda, K. S., Rai, S. N., Kumar, A. and Hossain, F. 2016. Rapid screening technique for evaluation of maize genotypes against stalk rot complex caused by Macrophomina phaseolina and Fusarium verticilloides. Indian J. Agric. Sci. 86: 1024-1030.
6 Gupta, G. K., Sharma, S. K. and Ramteke, R. 2012. Biology, epidemiology and management of pathogenic fungus Macrophomina phaseolina (Tassi) Goid with special reference to charcoal rot of soybean (Glycine max (L.) Merrill). J. Phytopathol. 160: 167-180.   DOI
7 Korea Meteorological Administration. 2020. URL https://data.kma.go.kr/stcs/grnd/grndTaList.do?pgmNo=70 [26 February 2020].
8 Korean Society of Plant Pathology. 2009. List of Plant Disease in Korea. 5th ed. Korean Society of Plant Pathology, Seoul, Korea. 853 pp.
9 Korea Statistical Information Service. 2020. URL http://kosis.kr/index/index.do [26 February 2020].
10 Short, G. E., Wyllie, T. D. and Bristow, P. R. 1980. Survival of Macrophomina phaseolina in soil and in residue of soybean. Phytopathology 70: 13-17.   DOI
11 Smith, G. S. and Wyllie, T. D. 1999. Charcoal rot. In: Compendium of Soybean Diseases, eds. by G. L. Hartman, J. B. Sinclair and J. C. Rupe, pp. 29-31. American Phytopathological Society, St. Paul, MN, USA.
12 Sun, S.-L., Zhu, Z.-D., Duan, C.-X., Zhao, P., Sun, F., Deng, D. et al. 2019. First report of charcoal rot caused by Macrophomina phaseolina on faba bean in China. Plant Dis. 103: 1415.
13 Sung, J. M., Park, J. H., Lee, S. C. and Chung, B. K. 1980. The outbreak and propagule formation of black root rot caused by Calonectria crotalariae in Korea. Korean J. Plant Prot. 19: 228-233. (In Korean)
14 Kaur, S., Dhillon, G. S., Brar, S. K., Vallad, G. E., Chand R. and Chauhan, V. B. 2012. Emerging phytopathogen Macrophomina phaseolina: biology, economic importance and current diagenostic trends. Crit. Rev. Microbiol. 38: 136-151.   DOI
15 Twizeyimana, M., Hill, C. B., Pawlowski, M., Paul, C. and Hartman, G. L., 2012. A cut-stem inoculation technique to evaluate soybean for resistance to Macrophomina phaseolina. Plant Dis. 96: 1210-1215.   DOI
16 Wrather, J. A., Anderson, T. R., Arsyad, D. M., Gai, J., Ploper, L. D., Porta- Puglia, A. et al. 1997. Soybean disease loss estimates for the top 10 soybean producing countries in 1994. Plant Dis. 81: 107-110.   DOI
17 Yu, S. H. and Park. J. S. 1980. Macrophomina phaseolina detected in seeds of Sesamum indicum and it's pathogenicity. Korean J. Plant Prot. 19: 135-140.
18 Yu, S. H. and Park, J. S. 1982. Survey on seed-borne fungi in some important crops of Korea. Res. Rep. Agric. Sci. Technol. Chungnam Natl. Univ. 9: 275-283. (In Korean)
19 Yum, K. J. and Park, E. W. 1989. Occurrence and distribution of soybean seed-borne fungi in Korea. Korean J. Plant Pathol. 5: 287-293. (In Korean)