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http://dx.doi.org/10.5423/RPD.2018.24.4.342

PCR Detection Method for Rapid Diagnosis of Bacterial Canker Caused by Clavibacter michiganensis on Tomato  

Park, Mi-Jeong (Horticultural and Herbal Crop Environment Division, National Institute of Horticultural and Herbal Science)
Back, Chang-Gi (Horticultural and Herbal Crop Environment Division, National Institute of Horticultural and Herbal Science)
Park, Jong-Han (Horticultural and Herbal Crop Environment Division, National Institute of Horticultural and Herbal Science)
Publication Information
Research in Plant Disease / v.24, no.4, 2018 , pp. 342-347 More about this Journal
Abstract
Bacterial canker caused by Clavibacter michiganensis is considered to be one of the most serious diseases, leading to economic damage to tomato worldwide. Diagnosis of the bacterial canker on tomato is known to be difficult because the causal pathogen is slow-growing on artificial media as well as causes latent infection in tomato. In this study, as a less time-consuming method, a specific primer set was newly designed for rapid detection of C. michiganensis. The method presented here is so simple, easy, and fast that it can be useful and practical in direct detection of the bacterial canker pathogen from tomato plants.
Keywords
Bacterial canker; Clavibacter michiganensis; Detection; PCR; Tomato;
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