Browse > Article
http://dx.doi.org/10.5423/RPD.2018.24.3.233

Development of a Multiplex RT-PCR for the Simultaneous Detection of Three Viruses in Cherry Plants  

Park, Chung Youl (Seed Testing & Research Center, Korea Seed & Variety Service)
Park, Jeongran (Seed Testing & Research Center, Korea Seed & Variety Service)
Lee, Geunsik (Seed Testing & Research Center, Korea Seed & Variety Service)
Yi, Seung-In (Seed Testing & Research Center, Korea Seed & Variety Service)
Kim, Byeong Hoon (Daegu Agricultural Technology Center)
Eom, Jung Sik (Daegu Agricultural Technology Center)
Lee, Som Gyeol (Daegu Agricultural Technology Center)
Kim, Hongsup (Seed Testing & Research Center, Korea Seed & Variety Service)
Publication Information
Research in Plant Disease / v.24, no.3, 2018 , pp. 233-236 More about this Journal
Abstract
A multiplex RT-PCR (mRT-PCR) assay was developed for the detection of the recently reported viruses, Cherry virus A (CVA), Little cherry virus 1 (LChV-1), and Little cherry virus 2 (LChV-2), in cherry plants in Korea. Eight sets of primers were designed for each virus and their specificity was tested by using various combinations of mixed primer sets. From the designed primer sets, one combination was selected and further evaluated to estimate the optimum temperature and detection limits of the mRT-PCR. A newly developed mRT-PCR assay was also tested using 20 cherry samples collected in the field. This mRT-PCR assay may be a useful tool for field surveys of diseases and the rapid detection of these three viruses in cherry plants.
Keywords
Cherry virus A; Little cherry virus 1; Little cherry virus 2; Multiplex RT-PCR;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Asano, S., Matsushita, Y., Hirayama, Y. and Naka, T. 2015. Simultaneous detection of Tomato spotted wilt virus, Dahlia mosaic virus and Chrysanthemum stunt viroid by multiplex RT-PCR in dahlias and their distribution in Japanese dahlias. Lett. Appl. Microbiol. 61: 113-120.   DOI
2 Cho, I. S., Choi, G. S. and Choi, S. K. 2013. Identification of Cherry green ring mottle virus on sweet cherry trees in Korea. Res. Plant Dis. 19: 326-330. (In Korean)   DOI
3 Cho, I. S., Choi, G. S., Choi, S. K., Seo, E. Y. and Lim, H. S. 2014. First report of Cherry necrotic rusty mottle virus infecting sweet cherry trees in Korea. Plant Dis. 98: 164.
4 Cho, S.-Y., Kim, H. and Yi, S.-I. 2018. First report of Little cherry virus 1 and 2 in sweet cherry in Korea. Plant Dis. 102: 1045.
5 Jo, Y., Lian, S., Cho, J. K., Chu, H., Choi, H. and Cho, W. K. 2017. First Report of Asian prunus virus 2 and Cherry virus A Infecting Japanese Apricot (Prunus mume) in Korea. Plant Dis. 101: 1683.
6 Kang, J. K. and Cho, G. R. 2007. Production situation and demand estimates of cherry (Prunus avium L.) in Korea. J. Korean Soc. Int. Agric. 19: 214-223. (In Korean)
7 Koinuma, H., Nijo, T., Iwabuchi, N., Yoshida, T., Keima, T., Okano, Y. et al. 2016. First complete genome sequence of Cherry virus A. Genome Announc. 4: e00498-16.
8 Lee, B.-C., Bae, J.-Y., Kim, S.-M., Ra, J.-E., Choi, N. J., Choi, M. Y. et al. 2017. Simultaneous detection of barley virus diseases in Korea. Res. Plant Dis. 23: 363-366. (In Korean)
9 Lee, J. S., Cho, W. K., Choi, H. S. and Kim, K. H. 2011. RT-PCR detection of five quarantine plant RNA viruses belonging to Poty and Tospoviruses. Plant Pathol. J. 27: 291-296.   DOI
10 Lee, S. Y., Yea, M. C., Back, C.G., Kang, I. K., Choi, C., Lee, S. H., et al. 2014a. Occurrence of cherry necrotic rusty mottle virus (CNRMV) and cherry green ring mottle virus (CGRMV) on sweet cherry. J. Fac. Agr. Kyushu U. 59: 1-4.
11 Lee, S. Y., Yea, M. C., Back, C. G., Choi, K. S., Kang, I. K., Lee, S. H. et al. 2014b. Survey of Cherry necrotic rusty mottle virus and Cherry green ring mottle virus incidence in Korea by Duplex RT-PCR. Plant Pathol. J. 30: 445-449.   DOI
12 Lim, S., Igori, D., Yoo, R. H., Zhao, F., Cho, I. S., Choi, G. S. et al. 2015. Genomic detection and characterization of a Korean isolate of Little cherry virus 1 sampled from a peach tree. Virus genes. 51: 260-266.   DOI
13 Lim, S., Igori, D., Baek, D., Cho, I. S., Choi, G. S. and Moon, J. S. 2017. First report of cherry virus A infecting Prunus mume in South Korea. Virusdisease 28: 220-221.   DOI
14 Mekuria, T. A., Smith, T. J., Beers, E., Watson, G. W. and Eastwell, K. C. 2013. Little cherry virus 2 is transmitted to sweet cherry by Pseudococcus maritimus (Ehrhorn), a new vector of this virus. Plant Dis. 97: 851.
15 Nam, M., Lee, Y. H., Park, C. Y., Lee, M. A., Bae, Y. S., Lim, S. et al. 2015. Development of multiplex RT-PCR for simultaneous detection of garlic viruses and the incidence of garlic viral disease in garlic genetic resources. Plant Pathol. J. 31: 90-96.   DOI
16 Raine, J., McMullen, R. D. and Forbes, A. R. 1986. Transmission of the agent causing little cherry disease by the apple mealybug Phenacoccus aceris and the dodder Cuscuta lupuliformis. Can. J. Plant Pathol. 8: 6-11.   DOI
17 Yao, B., Wang, G., Ma, X., Liu, W., Tang, H., Zhu, H. et al. 2014. Simultaneous detection and differentiation of three viruses in pear plants by a multiplex RT-PCR. J. Virol. Methods. 196: 113-119.   DOI
18 Yu, Y., Zhao, Z., Jiang, D., Wu, Z. and Li, S. 2013. A one-step multiplex RT-PCR assay for simultaneous detection of four viruses that infect peach. Lett. Appl. Microbiol. 57: 350-355.