Browse > Article
http://dx.doi.org/10.5423/RPD.2017.23.4.379

First Report of Cucumber mosaic virus Isolated from Sambungai (Gynura procumbens)  

Kwon, June (Department of Applied Biology, Kangwon National University)
Hong, Jin-Sung (Department of Applied Biology, Kangwon National University)
Publication Information
Research in Plant Disease / v.23, no.4, 2017 , pp. 379-382 More about this Journal
Abstract
In March 2016, an isolate of Cucumber mosaic virus (named Gyp-CMV) was isolated from the Sambungai (Gynura procumbens) showing the symptoms of mosaic and chlorosis. The isolate Gyp-CMV was characterized by disease reactions in several indicator plants, reverse transcription-polymerase chain reaction (RT-PCR), PCR-restriction fragment length polymorphism, and sequence analysis of movement protein (3a) and coat protein (CP) genes. Tobacco, tomato, pepper, ground cherry, and lambsquarters (Chenopodium quinoa and C. amaranticolor) appeared typical CMV symptoms, but zucchini and cucumber were not infected. Phylogenetic analysis of the 3a and CP gene indicated that Gyp-CMV belongs to the CMV subgroup II. Sequence identities of the Gyp-CMV 3a and CP genes showed 99.3% and 100% to that of Hnt-CMV at amino acid level. To our knowledge, this is the first report of CMV infection in Gynura procumbens.
Keywords
Coat protein; Cucumber mosaic virus; Gynura procumbens; RT-PCR;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Choi, J. K., Kwon, S. B., Lee, S. Y. and Park, W. M. 1990. Some properties of two isolates of Cucumber mosaic virus isolated from Aster yomena Makino and Commelina communis L. Plant Pathol. J. 6: 138-143.
2 Wahyuni, W. S., Dietzgen, R. G., Hanada, K. and Francki, R. I. B. 1992. Serological and biological variation between and within subgroup I and II strains of Cucumber mosaic virus. Plant Pathol. 41: 282-297.   DOI
3 Wang, Y., Gaba, V., Yang, J., Palukaitis, P. and Gal-On, A. 2002. Characterization of synergy between Cucumber mosaic virus and potyviruses in cucurbit hosts. Phytopathology 92: 51-58.   DOI
4 Kwak, H. R., Go, W. R., Kim, M., Kim, C. S., Choi, H. S., Seo, J. K., Kim, J.-G. and Kim, J. S. 2017. First report of Broad bean wilt virus 2 in Gynura procumbens in Korea. Plant Dis. 101: 514.
5 Choi, S. K., Choi, J. K., Park, W. M. and Ryu, K. H. 1999. RT-PCR detection and identification of three species of cucumoviruses with a genus-specific single pair of primers. J. Virol. Methods 83: 67-73.   DOI
6 Hassan, Z., Yam, M. F., Ahmad, M. and Yusof, A. P. M. 2010. Antidiabetic properties and mechanism of action of Gynura procumbens water extract in streptozotocin-induced diabetic rats. Molecules 15: 9008-9023.   DOI
7 Kim, J. S., Lee, S. H., Choi, H. S., Kim, M. K., Kwak, H. R., Kim, J. S., Nam, M., Cho, J.-D., Cho, I.-S. and Choi, G. S. 2012. 2007-2011 characteristics of plant virus infections on crop samples submitted from agricultural places. Res. Plant Dis. 18: 277-289. (In Korean)   DOI
8 Owen, J. and Palukaitis, P. 1988. Characterization of cucumber mosaic virus. I. Molecular heterogeneity mapping of RNA 3 in eight CMV strains. Virology 166: 495-502.   DOI
9 Palukaitis, P., Roossinck, M. J., Dietzgen, R. G. and Francki, R. I. B. 1992. Cucumber mosaic virus. Adv. Virus Res. 41: 281-348.
10 Roossinck, M. J., Zhang, L. and Hellwald, K. H. 1999. Rearrangements in the 5' nontranslated region and phylogenetic analyses of Cucumber mosaic virus RNA 3 indicate radial evolution of three subgroups. J. Virol. 73: 6752-6758.
11 Tomlinson, J. A. 1987. Epidemiology and control of virus diseases of vegetables. Ann. Appl. Biol. 110: 661-681.   DOI
12 Rosidah, Yam, M. F., Sadikun, A., Ahmad, M., Akowuah, G. A. and Asmawi, M. Z. 2009. Toxicology evaluation of standardized methanol extract of Gynura procumbens. J. Ethnopharmacol. 123: 244-249.   DOI