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http://dx.doi.org/10.5423/RPD.2017.23.3.241

Rapid Methods to Distinguish Heterodera schachtii from Heterodera glycines Using PCR Technique  

Ko, Hyoung Rai (Crop Protection Division, Department of Agro-food Safety and Crop Protection, National Institute of Agricultural Sciences)
Kim, Eun Hwa (Crop Protection Division, Department of Agro-food Safety and Crop Protection, National Institute of Agricultural Sciences)
Kim, Se Jong (2mbio Co., Ltd)
Lee, Jae Kook (Crop Protection Division, Department of Agro-food Safety and Crop Protection, National Institute of Agricultural Sciences)
Lee, Wang Hyu (Department of Agricultural Biology, Chonbuk National University)
Publication Information
Research in Plant Disease / v.23, no.3, 2017 , pp. 241-248 More about this Journal
Abstract
The purpose of this study was to develop rapid methods for distinguishing between Heterodera schachtii and H. glycines detected from chinese cabbage fields of highland in Gangwon, Korea. To do this, we performed PCR-RFLP and PCR with the primers set developed in this study for GC147, GC408 and PM001 population, H. schachtii, and YS224, DA142 and BC115 population, H. glycines. Eight restriction enzymes generated RFLP profiles of mtDNA COI region for populations of H. schachtii and H. glycines, repectively. As a result, treatment of two restriction enzymes, RsaI and HinfI, were allowed to distinguish H. schachtii from H. glycines based on the differences of DNA band patterns. The primer set, #JBS1, #JBG1 and #JB3R, amplified specific fragments with 277 and 339 bp of H. schachtii, 339 bp of H. glycines, respectively, while it did not amplify fragments from three root-knot nematodes and two root-lesion nematodes. Thus, the primer set developed in this study could be a good method, which is used to distinguish between H. schachtii and H. glycines.
Keywords
COI; Heterodera glycines; Heterodera schachtii; PCR-RFLP; Species specific primer;
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