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http://dx.doi.org/10.5423/RPD.2017.23.2.193

Specific Primer Sets for RT-PCR Detection of Major RNA Viruses of Tomato Plants in Korea  

Shin, Jun-Sung (BioCube System, Inc.)
Han, Jung-Heon (Gene Engineering Division, National Institute of Agricultural Sciences, Rural Development Administration)
Shin, Yu-Ju (BioCube System, Inc.)
Kwak, Hae-Ryun (Crop Protection Division, National Institute of Agricultural Sciences, Rural Development Administration)
Choi, Hong-Soo (Crop Protection Division, National Institute of Agricultural Sciences, Rural Development Administration)
Kim, Jeong-Soo (Department of Plant Medicals, Andong National University)
Publication Information
Research in Plant Disease / v.23, no.2, 2017 , pp. 193-201 More about this Journal
Abstract
Major tomato viruses in Korea are Tomato chlorosis virus (ToCV), Tomato spotted wilt virus (TSWV), Cucumber mosaic virus (CMV), Pepper mottle virus (PepMoV), and Tomato mosaic virus (ToMV). RT-PCR conditions for the viruses were examined, especially in primer set and RT-PCR mixture. Total 46 primer sets from the unique sequence of the viruses were tested for nonspecific background products in a RT-PCR mixture without template. Among them 16 primer sets were applied to healthy tomato RNA, resulting the compatibility between RT-PCR mixture and primer set influenced RT-PCR to reduce nonspecific background products. Based on the combinations among cDNA synthesis parameters and RT-PCR mixtures, two reaction mixtures were finally selected for ToCV detection. The condition allowed to determine more specific primer sets; C029 (ToCV), C072 (TSWV), C070 (CMV), C048 (PepMoV), and C065 (ToMV). These primer sets are expected to be of use to specific detection of the major viruses in tomato plants.
Keywords
Detection; Primer; RT-PCR; Tomato; Virus;
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