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http://dx.doi.org/10.5423/RPD.2016.22.4.257

Illustration of Disease Suppression of Anthracnose on Cucumber Leaves by Treatment with Chlorella fusca  

Lee, Yun Ju (Major of Plant Resources and Environment, Faculty of Bioscience and Industry, College of Applied Life Sciences, Jeju National University)
Ko, Yun Jung (Major of Plant Resources and Environment, Faculty of Bioscience and Industry, College of Applied Life Sciences, Jeju National University)
Jeun, Yong Chull (Major of Plant Resources and Environment, Faculty of Bioscience and Industry, College of Applied Life Sciences, Jeju National University)
Publication Information
Research in Plant Disease / v.22, no.4, 2016 , pp. 257-263 More about this Journal
Abstract
Chlorella is known as chlorophyceae which can live autotrophically by photosynthesis, promote the growth of plants and suppress some plant diseases. However, a few researches in inhibition mechanism of plant diseases by chlorella have been carried out. In this study cucumber leaves pre-treated with Chlorella fusca suspension were investigated whether anthracnose by Colletotrichum orbiculare is suppressed or not. Furthermore, in order to illustrate how the algae can restrain the antracnose, the infection structures of C. orbiculare were observed on the cucumber leaves pre-treated with the algae. Consequently, appressorium formation rate was apparently reduced in the cucumber leaves pre-treated with C. fusca compared to untreated control one. Also, the numbers of conidia found at the inoculation sites were significantly reduced compared to untreated one. On the other hand, on the leaves pre-treated with $Benomyl^{(R)}$ appressorium formation were decreased remarkably and numbers of conidia were also reduced similar with those pre-treated with C. fusca. Based on these results, it was revealed that occurrence of anthracnose can be suppressed by C. fusca pre-treatment and suggested that biochmical or structural hinderance by C. fusca resulting in the decline of appressorium formation on the leave surfaces may play an important role in the disease suppression.
Keywords
Algae; Biofungicide; Colletotrichum orbiculare; Fluorescence microscopy; Infection structures;
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