Browse > Article
http://dx.doi.org/10.5423/RPD.2016.22.3.194

Complete Sequence Analysis of a Korean Isolate of Chinese Yam Necrotic Mosaic Virus and Generation of the Virus Specific Primers for Molecular Detection  

Kwon, Sun-Jung (Department of Horticultural Environment, National Institute of Horticultural and Herbal Science, Rural Development Administration)
Cho, In-Sook (Department of Horticultural Environment, National Institute of Horticultural and Herbal Science, Rural Development Administration)
Choi, Seung-Kook (Department of Horticultural Environment, National Institute of Horticultural and Herbal Science, Rural Development Administration)
Yoon, Ju-Yeon (Department of Horticultural Environment, National Institute of Horticultural and Herbal Science, Rural Development Administration)
Choi, Gug-Seoun (Department of Horticultural Environment, National Institute of Horticultural and Herbal Science, Rural Development Administration)
Publication Information
Research in Plant Disease / v.22, no.3, 2016 , pp. 194-197 More about this Journal
Abstract
Chinese yam necrotic mosaic virus (CYNMV) is one of the most widespread viruses in Chinese yam (Dioscorea opposita Thunb.) and causes serious yield losses. Currently, genetic information of CYNMV is very restricted and complete genome sequences of only two isolates (one from Japan and another from China) have been reported. In this study, we determined complete genome sequence of the CYNMV isolate AD collected from Andong, Korea. Genetic analysis of the polyprotein amino acid sequence revealed that the Korean isolate AD has high similarity with the Japanese isolate PES3 (97%) but relatively low similarity with the Chinese isolate FX1 (78%). Phylogenetic analysis using the CYNMV 3' proximal nucleotide sequences harboring the coat protein and 3' untranslated region further supported genetic relationship among the CYNMV isolates. Based on comparative analysis of the CYNMV genome sequences determined in this study and other previous studies, we generated molecular detection primers that are highly specific and efficient for CYNMV diagnosis.
Keywords
Chinese yam; Chinese yam necrotic mosaic virus; Complete genome; Genetic analysis;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Fukumoto, F. and Tochihara, H. 1978. Chinese yam necrotic mosaic virus. Ann. Phytopathol. Soc. Jpn. 44: 1-5.   DOI
2 Kang, D. K., Kondo, T., Shin, J. H., Shin, H. Y., Sung, J. H., Kang, S. G. and Chang, M. U. 2003. Chinese yam necrotic mosaic virus isolated from Chinese yam in Korea. Res. Plant Dis. 9: 107-115. (In Korean)   DOI
3 Kondo, T. 2001a. The 3'-terminal sequence of Chinese yam necrotic mosaic virus genomic RNA: a close relationship with macluravirus. Arch. Virol. 146: 1527-1535.   DOI
4 Kondo, T. 2001b. Detection of Chinese yam necrotic mosaic virus by RT-PCR. Jpn. J. Phytopathol. 66: 263.
5 Kondo, T. and Fujita, T. 2012. Complete nucleotide sequence and construction of an infectious clone of Chinese yam necrotic mosaic virus suggest that macluraviruses have the smallest genome among members of the family Potyviridae. Arch. Virol. 157: 2299-2307.   DOI
6 Kondo, T., Kang, D. K., Fuji, S. and Chang, M. U. 2003. Sequence diversity in the coat protein and 3"-untranslated region of Chinese yam necrotic mosaic virus RNA. J. Gen. Plant Pathol. 69: 397-399.   DOI
7 Kondo, T., Kasai, K., Yamashita, K. and Ishitani, M. 2007. Selection and discrimination of an attenuated strain of Chinese yam necrotic mosaic virusfor cross-protection. J. Gen. Plant Pathol. 73: 152-155.   DOI
8 Kondo, T., Kogawa, K. and Ito, K. 2015. Evaluation of cross protection by an attenuated strain of Chinese yam necrotic mosaic virus in Chinese yam. J. Gen. Plant Pathol. 81: 42-48.   DOI
9 Kwon, S. J., Cho, I. S., Yoon, J. Y., Choi, S. K. and Choi, G. S. 2016. First report of Broad bean wilt virus 2 in Dioscorea opposita Thunb. in Korea. Plant Dis. 100: 538.
10 Kwon, S. J., Tan, S. H. and Vidalakis, G. 2014. Complete nucleotide sequence and genome organization of an endornavirus from bottle gourd (Lagenaria siceraria) in California, U.S.A. Virus Genes 49: 163-168.   DOI
11 Tamura, K., Stecher, G., Peterson, D., Filipski, A. and Kumar, S. 2013. MEGA6: molecular evolutionary genetic analysis version 6.0. Mol. Biol. Evol. 30: 2725-2729.   DOI