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http://dx.doi.org/10.5423/RPD.2016.22.3.178

Reverse Transcription Loop-Mediated Isothermal Amplification Assay for Rapid Detection of Soybean yellow mottle mosaic virus  

Bae, Dae Hyeon (School of Applied Biosciences, Kyungpook National University)
Park, Chung Youl (School of Applied Biosciences, Kyungpook National University)
Kim, Bong-Sub (School of Applied Biosciences, Kyungpook National University)
Lee, Yeong-Hoon (Crop Production Technology Research Division, Department of Southern Area Crop Science, National Institute of Crop Science, Rural Development Administration)
Yoon, Young-Nam (Crop Production Technology Research Division, Department of Southern Area Crop Science, National Institute of Crop Science, Rural Development Administration)
Kang, Hang Won (Crop Production Technology Research Division, Department of Southern Area Crop Science, National Institute of Crop Science, Rural Development Administration)
Oh, Jonghee (School of Applied Biosciences, Kyungpook National University)
Lee, Su-Heon (School of Applied Biosciences, Kyungpook National University)
Publication Information
Research in Plant Disease / v.22, no.3, 2016 , pp. 178-183 More about this Journal
Abstract
Soybean yellow mottle mosaic virus (SYMMV) is a new emerging plant virus detected in soybean (Glycine max) in Korea. Reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for rapid detection of SYMMV has been developed. In this study, we have designed primers (SYMM-F3/B3/FIP/BIP) specific to sequences from the coat protein gene of SYMMV genome. Sensitivity analysis showed that RT-LAMP was 10 to 100 times more sensitive than reverse transcription polymerase chain reaction (RT-PCR). The optimal reaction condition of RT-LAMP was determined at $65^{\circ}C$ for 50 minutes. The result indicates that RT-LAMP assay does not require special equipment and long time for SYMMV detection. Therefore, it can be an alternative detection method of RT-PCR in laboratory.
Keywords
Soybean; Soybean yellow mottle mosaic virus; Reverse transcription loop-mediated isothermal amplification;
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