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http://dx.doi.org/10.5423/RPD.2016.22.1.1

Observation of Growth Inhibition of Elsinoe fawcettii on Satsuma Mandarin Leaves Pre-treated with Rhizobacterial Strains by a Scanning Electron Microscope  

Park, Jae Sin (Major of Plant Resources & Environment, College of Applied Life Sciences, Jeju National University)
Song, Min-A (Major of Plant Resources & Environment, College of Applied Life Sciences, Jeju National University)
Jeun, Yong Chull (Major of Plant Resources & Environment, College of Applied Life Sciences, Jeju National University)
Publication Information
Research in Plant Disease / v.22, no.1, 2016 , pp. 1-8 More about this Journal
Abstract
Elsinoe fawcettii causing citrus scab was suppressed by rhizobacterial strains such as Burkholderia gladioli MRL408-3, TRH423-3 and Pseudomonas fluorescens THJ609-3, TRH415-2 having antifungal activity. The leaf surface of Satsuma mandarin, which was pre-treated with the rhizobacterial strains, was observed by a scanning electron microscope (SEM) after inoculation with E. fawcettii. The number of lesions was reduced on the leaves pre-treated with the rhizobacterial strains compared to those of untreated leaves. Especially, the lesions numbers was apparently reduced on the leaves pre-treated with B. gladioli MRL408-3. The observation by SEM revealed that not only the germination rate but also the length of germ tube of the pathogen were decreased on the rhizobacterial strains pre-treated leaves. These inhibition of the fungal growth was more strongly expressed on the leaves pre-treated with commercial fungicide imibenconazole, by which the lesions was rarely found on the leaves. Based on these results, it was suggested that rhizobacterial strains may inhibit the germination and growth of the E. fawcettii on the surface of citrus leaves, resulting in decrease of disease severity.
Keywords
Antibiotic; Bio-fungicide; Biological control; Citrus scab; Ultrastructure;
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1 Bae, Y. S., Park, K. S. and Choi, O. H. 2007. Laboratory culture media-dependent biocontrol ablity of Burkholdria gladioli strain B543. Plant Pathol. J. 23: 161-165.   DOI
2 Beneduzi, A., Ambrosini, A. and Passaglia, L. M. P. 2012. Plant growth-promoting rhizobacteria (PGPR): their potential as antagonists and biocontrol agents. Genet. Mol. Biol. 35(4 Suppl): 1044-1051.   DOI
3 Bolwerk, A., Lagopodi, A. L., Wijfjes, A. H., Lamers, G. E., Chin-A-Woeng, T. F., Lugtenberg, B. J. and Bloemberg, G. V. 2003. Interactions in the tomato rhizosphere of two Pseudomonas biocontrol strains with the phytopathogenic fungus Fusarium oxysporum f. sp. radicis-lycopersici. Mol. Plant Microbe Interact. 16: 983-993.   DOI
4 Cho, J. I., Cho, J. Y., Park, Y. S., Yang, S. Y. and Heo, B. G. 2007. Selection and identification of actinomyces, and its biological control effect against the bacterial blossom blight infected in kiwifruits. Korean J. Hortic. Sci. Technol. 25: 235-240.
5 David, M. W. 1988. Biological control of soilborne plant pathogens in the rhizosphere with bacteria. Annu. Rev. Phytopathol. 26: 379-407.   DOI
6 de Weert, S., Kuiper, I., Lagendijk, E. L., Lamers, G. E. and Lugtenberg, B. J. 2004. Role of chemotaxis toward fusaric acid in colonization of hyphae of Fusarium oxysporum f. sp. radicis-lycopersici by Pseudomonas fluorescens WCS365. Mol. Plant Microbe Interact. 17: 1185-1191.   DOI
7 Elad, Y. and Kapat, A. 1999. The role of Trichoderma harzianum protease in the biocontrol of Botrytis cinerea. Eur. J. Plant Pathol. 105: 177-189.   DOI
8 Hayat, M. A. 1989. Principles and Techniques of Electron Microscopy: Biologacal Applications. 3rd ed. The Macmillan Press, Boca Raton, FL, USA. 1-78 pp.
9 Hyun, J. W., Timmer, L. W., Lee, S. C., Yun, S. H., Ko, S. W. and Kim, K. S. 2001. Pathological characterization and molecular analysis of Elsinoe isolates causing scab diseases of citrus in Jeju isolates in Korea. Plant Dis. 85: 1013-1017.   DOI
10 Kamilova, F., Lamers, G. and Lugtenberg, B. 2008. Biocontrol strain Pseudomonas fluorescens WCS365 inhibits germination of Fusarium oxysporum spores in tomato root exudate as well as subsequent formation of new spores. Environ. Microbiol. 10: 2455-2461.   DOI
11 Kim, Y. K., Hong, S. J., Shim, C. K., Kim, M. J., Choi, E. J., Lee, M. H., Park, J. H., Han, E. J., An, N. H. and Jee, H. J. 2012. Functional analysis of Bacillus subtilis isolates and biological control of red pepper powdery mildew using Bacillus subtilis R2-1. Res. Plant Dis. 18: 201-209. (In Korean)   DOI
12 Kang, S. Y. and Jeun, Y. C. 2012. Suppression effect of white rot by bacterial isolates from plant rhizosphere in garlic plants. Korean J. Organic Agri. 28: 25-32. (In Korean)
13 Kim, S. G., Zakaullah, K., Jeon, Y. H. and Kim, Y. H. 2009. Inhibitory effect of Paenibacillus polymyxa GBR-462 on Phytophthora capsici causing phytophthora blight in chili pepper. J. Phytopathol. 157: 329-337.   DOI
14 Kim, S. Y., Hyun, J. W. and Jeun, Y. C. 2011. Suppression effect and mechanism of citrus scab in the citrus pre-inoculated with rhizobacterial strains. Res. Plant Dis. 17: 302-310. (In Korean)   DOI
15 Kloepper, J. W., Ryu, C. M. and Zhang, S. 2004. Induced systemic resistance and promotion of plant growth by Bacillus spp. Phytopathology 94: 1259-1266.   DOI
16 Ko, Y. J., Kang, S. Y. and Jeun, Y. C. 2012. Suppression of citrus melanose on the leaves treated with rhizobacterial strains after inoculation with Diaporthe citri. Res. Plant Dis. 18: 331-337.   DOI
17 Lahlali, R., Peng, G., McGregor, L., Gossen, B. D., Hwang, S. F. and McDonald, M. 2011. Mechanisms of the biofungicide Serenade (Bacillus subtilis QST713) in suppressing clubroot. Biocontrol Sci. Technol. 21: 1351-1362.   DOI
18 Manjula, K., Krishna Kishore, G., Girish, A. G. and Singh, S. D. 2004. Combined application of Pseudomonas fluorescens and Trichoderma viride has an improved biocontrol activity against stem rot in groundnut. Plant Pathol. J. 20: 75-80.   DOI
19 Park, K. S. 2000. Biological control of vegetable diseases by rhizocbacteria. Korean J. Organic Agri. 6: 93-105.
20 Nam, M. H., Choi, J. P., Kim, H. J., Lee, J. J., Lim, K. H., Kim, Y. G., Kim, H. T. and Jeun, Y. C. 2010. Controlling activity of Bacillus subtilis KB-401 against cucumber powdery mildew caused by Sphaerotheca fusca. Korean J. Pestic. Sci. 14: 49-53.
21 Raaijmakers, J. M., Weller, D. M. and Thomashow, L. S. 1997. Frequency of antibiotic-producing Pseudomonas spp. in natural environments. Appl. Environ. Microbiol. 63: 881-887.
22 Rangarajan, S., Saleena, L. M., Vasudevan, P. and Nair, S. 2003. Biological suppression of rice disease by Pseudomonas spp. under saline soil conditions. Plant Soil 251: 73-82.   DOI
23 Raupach, G. S. and Kloepper, J. W. 1998. Mixtures of plant growth-promoting rhizobacteria enhance biological control of multiple cucumber pathogens. Phytopathology 88: 1158-1164.   DOI
24 Song, M., Yun, H. Y. and Kim, Y. H. 2014. Antagonistic Bacillus species as a biological control of ginseng root rot caused by Fusarium cf. incarnatum. J. Ginseng Res. 38: 136-145.   DOI
25 Tian, S. P., Fan, Q., Xu, Y. and Jiang, A. L. 2002. Effects of calcium on biocontrol activity of yeast antagonists against the postharvest fungal pathogen Rhizopus stolonifer. Plant Pathol. 51: 352-358.   DOI
26 Timmer, L. W., Priest, M., Broadbent, P. and Tan, M.-K. 1996. Morphological and pathological characterization of species of Elsinoe causing scab diseases of citrus. Phytopathology 86: 1032-1038.   DOI
27 Validov, S. Z., Kamilova, F. and Lugtenberg, B. J. J. 2009. Pseudomonas putida strain PCL1760 controls tomato foot and root rot in stonewool under industrial conditions in a certified greenhouse. Biol. Control 48: 6-11.   DOI
28 Yang, J. S., Kang, S. Y. and Jeun Y. J. 2014. Suppression of citrus canker by pretreatment with rhizobacterial strains showing antibacterial activity. Res. Plant Dis. 20: 101-106. (In Korean)   DOI
29 Weller, D. M. 1988. Biological control of soilborn plant pathogens in the rhizosphere with bacteria. Ann. Rev. Phytopathol. 26: 379-407.   DOI
30 Wisniewski, M., Droby, S., Chalutz, E. and Eilam, Y. 1995. Effects of Ca2+ and Mg2+ on Botrytis cinerea and Penicillium expansum in vitro and on the biocontrol activity of Candida oleophila. Plant Pathol. 44: 1016-1024.   DOI