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http://dx.doi.org/10.5423/RPD.2015.21.2.058

Control of Powdery Mildew by Foliar Application of a Suspension of Cheonggukjang  

Kim, Min-Jeong (Organic Agriculture Division, National Academy of Agricultural Science, Rural Development Administration)
Shim, Chang-Ki (Organic Agriculture Division, National Academy of Agricultural Science, Rural Development Administration)
Kim, Yong-Ki (Organic Agriculture Division, National Academy of Agricultural Science, Rural Development Administration)
Hong, Sung-Jun (Organic Agriculture Division, National Academy of Agricultural Science, Rural Development Administration)
Park, Jong-Ho (Organic Agriculture Division, National Academy of Agricultural Science, Rural Development Administration)
Han, Eun-Jung (Organic Agriculture Division, National Academy of Agricultural Science, Rural Development Administration)
Jee, Hyeong-Jin (Organic Agriculture Division, National Academy of Agricultural Science, Rural Development Administration)
Kwon, Jin-Hyeuk (Eco-friendly Research Department, Gyeongsangnam-do Agricultural Research & Extension Services)
Kim, Seuk-Chul (Organic Agriculture Division, National Academy of Agricultural Science, Rural Development Administration)
Publication Information
Research in Plant Disease / v.21, no.2, 2015 , pp. 58-66 More about this Journal
Abstract
This study was conducted to evaluate control efficacy of a fermented food 'Cheonggukjang' against cucumber powdery mildew caused by Sphaerotheca fuliginea in greenhouse. Sterilized Daepung beans were inoculated with the rice straw as natural inoculum and then incubated for 72 hrs at $42^{\circ}C$ in the household cheonggkjang maker. After 72 hrs of cheonggukjang fermentation, white zymogens were grown on the surface of a sterile Daepung beans. The pH of the 72 hrs fermented soybean was not significantly changed and electrical conductivity was found to increase by about 2 times than before fermentation. The population density of soybean zymogen showed a peak of growth at 60 hrs after fermentation and the concentration of zymogen was $8.2{\times}10^7cfu/ml$. Soybean zymogen form of the colony was divided into three kinds of bacteria and a white and a large colony (WL) was predominant bacteria among those up to 60 hrs of fermentation. To control the cucumber powdery mildew, diluted solutions of cheonggukjang was applied from 6.0% to 30.0% on cucumber leaves and they showed injury symptoms on cucumber leaves in more than 15% of them. However, more than 6.0% diluted cheonggukjang solutions showed more than 77.8% control effect of cucumber powdery mildew at 15 days after treatment. The fermented bacteria of Chenggukjang were well established in the cucumber leaf area at 15 days after treatment. The antifungal activity of 10% diluted cheonggukjang solutions was excellent for four species of plant fungal pathogens, Colletotrichum gloeosporioides, Sclerotinia cepivorum, Rhizoctonia sloani and Phytophthora capsici in the dual culture test. Results indicated that foliar application of Cheonggukjang solution could be used for the control of powdery mildews occurring on organically cultivated cucumber.
Keywords
Antifungal activity; Cucumber; Cheonggukjang; Sphaerotheca fuliginea;
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1 Yi, D. H., Lee, N. W. and Kwon, T. J. 2003. Purification and chaeacterization of an antifungal antibiotic from Bacillus subtilis LAM 97-44. J. Korean Soc. Agr. Chem. Biotechnol. 46: 69-73.
2 Yoo, J. Y. 1997. Present status of industries and research activities of Korean fermented soybean products. Korean J. Appl. Microbiol. Biotechnol. 23:13-30.
3 Yoo, S. K., Cho, S. H., Kang, S. M. and Lee S. H. 1999. Isolation and identification of microorganisms in Korean traditional soybean paste and soybean sauce. Korean J. Appl. Microbiool. Biotechnol. 27: 113-117.
4 Yoo, S. M. and Chang, C. M. 1999. Study on the processing adaptability of soybean cultivars for Korean traditional Cheonggukjang preparation. J. Korean Sco. Agr. Chem. Biotechnol. 42: 91-98.
5 Yoon, K. C., Kim, D. H., Kim, J. O., Park, B. J., Yook, H. S., Cho, J. M. and Byun, M. W. 2001. Quality characteristics of the Chungkookjang fermented by the mixed culture of Bacillus natto and B. licheniformis. J. Korean Soc. Food Sci. Nutr. 31: 204-210.
6 Belanger, R. R., Dik, A. J. and Menzies, J. M. 1998. Powdery mildew recent advances toward intergrated controil In: Plant-microbe Interactions and Biological Control, ed. by G. J. Boland and D. L. Kukendall, pp. 89-126. Marcel dekker, New York, USA.
7 Cladera-Olivera, F., Caron, G. R. and Brandelli, A. 2004. Bacterocin-like substance production by Bacillus licheniformis strain P40. Lett. Appl. Microbiol. 38: 251-256.   DOI
8 Braun, U., Cook, T. A., Inman, A. J. and Shin, H. D. 2002. The taxonomy of the powdery mildew fungi. In: The Powdery Mildews: A Comprehensive Treatise, ed. by R. R. Belanger, W. R. Bushnell, A. J. Dik and T. L. W. Carver, pp. 13-55. APS Press, St. Paul, MN, USA.
9 Chang, J. Y., Lee, H. H., Kim, I. C. and Chang, H. C. 2001. Characterzation of bacteriocin produced by Bacillus licheniforms cy2. J. Korean Soc. Food Sci. Nutr. 30: 410-414.
10 Choi, U. K., Son, D. H., Ji, W. D., Im, M. H., Choi, J. D. and Chung, U. G. 1998. Changes of taste components and palatability during chunggukjang fermentation by Bacillus subtilis DC-2. J. Korean Food Sci. Nutr. 27: 840-845.
11 Copping, L. G. 2009. The manual of biocontrol agents. 3th ed. pp. 702. eds by BCPC, UK.
12 Endo, Y. 1989. Studies on the life-cycle of cucurbit powdery mildew fungus Sphaerotheca fuliginea (schlecht) Poll. Spec. Bull. Fukushima Pre. Agr. Exp. Stn. 5: 1-106.
13 Fravel, D. R. 2005. Commercialization and implementation of biocontrol. Annu. Rev. Phytopathol. 43: 337-359.   DOI
14 Fujii, H., Shiraishi, A., Kaba, H., Shibagaki, M., Takahashi, S. and Honda, A. 1975. In topics in seceondary metabolism I Bacillus subtillis: Molecular biology and industrial application. Hakkokogaku Kaishi 53: 424.
15 He, L., Chen, W. and Liu, Y. 2006. Production and partial characterization of bacteriocin-like peptides by Bacillus licheniformis ZJU12. Microbiol. Res. 161: 321-326.   DOI
16 Jarvis, W. R., Gubler, W. D. and Grove, G. G. 2002. Epidemiology of powdery mildews in agricultural pathosystems, In: The Powdery Mildews: A Comprehensive Treatise, ed. by Belanger, R. R., Bushnell, W. R., Dik, A. J., Carver, T. L. W., pp. 169-199. APS Press, St. Paul, MN, USA.
17 Hijwegan, T. 1992. Biological control of cucumber powdery mildew with Tilletiopsis minor under greenhouse conditions. Neth. J. Plant Pathol. 98: 221-225.   DOI
18 Hong, S. W., Lim. I. K., Kim, Y. W., Shin, S. M. and Chung, K. S. 2013. Denaturing gradient gel electrophoresis and culture-based analysis of the bacterial community in Cheonggukjang, a Korean traditional fermented soybean food from Gangwon province. Korean J. Food Sci. Technol. 45: 515-520.   DOI   ScienceOn
19 Hwang, J. Y., Shim, C. K., Ryu, K. Y., Choi, D. H. and Jee, H. J. 2006. Selection of Brevibacillus brevis B23 and Bacillus stearothrmophilus B42 as biological control agents against Sclerotinia rot of lettuce. Res. Plant Dis. 12: 254-259. (In Korean)   DOI
20 Jee, H. J., Shim, C. K., Ryu, K. Y., Lee, B. M., Park, J. H. and Choi, D. H. 2006. Effects of cooking oils on control of powdery mildew of cucumber caused by Spaerotheca fuliginea. Plant Pathol. J. 21: 415.
21 Kang, K. J., Park, H. H. and Cho, J. I. 2000. Control of Aflatoxin and characteristics of the quality in Doenjang (soybean paste) prepared with antifungal bacteria. Korean J. Food Sci. Technol. 32: 1258-1265.
22 Kang, Y. M., Chang, K. M., Lee, J. J., Han, K. P., Hong, J. H. and Jeon, H. K. 2006. Effect of cheongguk-jang byproduct (non-fermented soybean) on chemical properties of soil and growth of lettuce. 2006 International Symposium and Poster Presentation in Korean Organic Resource Recycling Association. pp. 217-222.
23 Kim, J. K., Shim, C. K., Park, S. W., Park, B. J., Jee, H. J., Kim, W. I., Kwon, O. K. and Im, G. J. 2009. Control of powdery mildew of cucumber by using Mayonnaise. Korean J. Organic Agric. 17: 557-566.
24 Kim Y. K., Hong, S. J., Jee, H. J., Shim, C. K., Park, J. H., Han, E. J., An, N. H. Lee, S. D. and Yoo, J. H. 2011. Population dynamics of effective microorganisms in microbial pesticides and environmental-friendly organic materials according to storing period and temperature. Korean J. Pesticide Sci. 15: 55-60.
25 Kim, C. H., Jee, H. J., Park, K. S. and Lee, E. J. 1990. Studies on the biological control of phytophyhora blight of red-pepper. V. Performance of antagonistic agent in fields. Korean J. Phythopath. 6: 201-206.
26 Kim, G. H., Park, J. Y., Cha, J. H., Jeon, C. S., Hong, S. J., Kim, Y. K., Hun, J. S. and Koh, Y. J. 2011. Control effect of major fungal diseases of cucumber by mixing of biofungicides registered for control of powdery mildew with other control agents. Korean J. Pesticide Sci. 15: 323-328.
27 Kim, J. Y., Hong, S. S., Lee, J. G., Park, K. Y., Kim, H. G. and Kim, J. W. 2006. Determinants of economic threshold for powdery mildew on cucumber. Res. Plant Dis. 12: 231-234.   DOI
28 Kim, J. Y., Hong, S. S., Lim, J. W. Park, K. Y. and Kim, H. G. 2008. Screening of fungicide resistance of cucumber powdery mildew pathogens, Sphaerotheca fusca in gyeonggi province. Res. Plant Dis. 14: 95-101. (In Korean)   DOI   ScienceOn
29 Kim, Y. S., Jung, H. J., Park, Y. S. and Yu, T. S. 2003. Characteristics of flavor and functionally of Bacillus subtilis K-20 chunggukjang. Korean J. Food Sci. Technol. 35: 475-478.
30 Kwon, E. Y. 2000. Standardization of chunggukjang preparation and its cancer preventive effect. MS thesis. Pusan Natl. Univ. Pusan, Korea. 78 pp.
31 Lee, M. H. and Kim, Y. S. 2014. Control of powdery mildew on cucumber by using oleic acid in the greenhouse. Korean J. Organic Agri. 22: 695-703.   DOI
32 Kwon, H. Y., Kim, Y. S., Kwon, G. S., Kwon, C. S. and Sohn, H. Y. 2004. Isolation of immune-stimulating strain Bacillus pumilus JB-1 from cheonggukjang and fermentation al characteristics of JB-1 Korean J. Microbiol. Biotechnol. 3: 291-296.
33 Kwon, H. Y., Lee, H. A., Park, J. Y., Kim, J. S., Lim, J. K., Park, C. S., Kwon, D. Y., Kim, Y. S. and Kim, J. H. 2009. Development of a RAPDPCR method for identification of Bacillus species isolated from cheonggukjang. Int. J. Food Microbiol. 129: 282-287.   DOI
34 Lee, B. Y., Lim, D. M. and Kim, K. H. 1991. Physico-chemical properties of viscous substance extracted from chungkookjang. Korean J. Food Sci. Technol. 23: 599-604.
35 Lee, M. Y., Park, S. Y., Jung, K. O., Park, K. Y. and Kim, S. D. 2005. Quality and functional characteristics of cheonggukjang prepared with various Bacillus sp. isolated from traditional cheonggukjang. J. Food Sci. 70: M191-M196.
36 Lee, S. H., Baek, L. M. and Park, L. Y. 2008. Physiological characteristics of Bacillus spp. Isolated from rice straw as cheonggukjang starter. Korean J. Food Sci. Technol. 40: 562-567.
37 Lee, S. Y., Kim, Y. K. and Kim, H. G. 2007. Biological control of cucumber powdery mildew using a hyperparasite, Ampelomyces quisqualis 94013. Res. Plant Dis. 13: 197-203. (In Korean)   DOI
38 Lee, S. Y., Weon, H. Y., Kim, J. J., Han, J. H. and Kim, W. G. 2013. Biological control of cucumber powdery mildew by Bacillus amyloliquefaciens M27. Korean J. Mycol. 41: 268-273.   DOI
39 Nam, M. H., Choi, J. P., Kim, H. J., Lee, J. J., Lim, K. H., Kim, Y. G., Kim, H. T. and Jeun, Y. C. 2010. Controlling activity of Bacillus subtillus KB-401 against cucumber powdery mildew caused by Sphaerotheca fusca. Korean J. Pesticide Sci. 14: 49-53.
40 Moon, H. K., Lee, S. W., Moon, J. N., Kim, D. H., Yoon, W. J. and Kim, G. Y. 2011. Quality characteristics of various beans in distribution. J. East Asian Soc. Dietary Life 21: 215-221.
41 Okanmato, A., Hanagata, H., Matsumoto, E., Kawamura, T., Koizume, Y. and Yanagida, F. 1995. Angiotensin I converting enzyme inhibitory activities of various fermented foods. Biosci. Biotech. Bioch. 59: 1147-1149.   DOI
42 Park, S. M., Kim, H. S. and Yu, T. S. 2006. Antifungal activity of Bacillus sp. KUM-101 against gray mold Botrytis cinerea. Korean J. Microbiol. Biotechnol. 34: 63-69.
43 Romero, D., Perez-Garcia, A., Rivera, M. E., Cazola, F. M. and de Vicente, A. 2004. Isolation and evaluation of antagonistic bacteria towards the cucurbit powdery mildew fungus Podosphaera fusca. Appl. Microbiol. Biotechnol. 64: 263-269.   DOI
44 Shon, M. Y., Seo, K. L., Lee, S. W., Choi, S. H. and Sung, N. J. 2000. Biological activities of chungkugjang prepared with black bean and changes in phytoestrogen content during fermentation. Korean J. Food Sci. Technol. 32: 936-941.
45 The Korean Society of Plant Pathology. 2009. List of plant diseases in Korea, 5th edition, pp. 853. JY press, Anyang, Korean.
46 Wei, Q., Wolf-Hall, C. and Chang, K. C. 2001. Natto characteristics as affected by steaming time, Bacillus strain, and fermentation time. J. Food Sci. 66: 167-173.   DOI