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http://dx.doi.org/10.5423/RPD.2014.20.4.307

Characterization of Brugmansia mosaic virus Isolated from Brugmansia spp. in Korea  

Park, Chung Youl (School of Applied Biosciences, Kyungpook National University)
Kim, Bong-Sub (School of Applied Biosciences, Kyungpook National University)
Nam, Moon (School of Applied Biosciences, Kyungpook National University)
Lee, Min-A (School of Applied Biosciences, Kyungpook National University)
Baek, Da-Som (School of Applied Biosciences, Kyungpook National University)
Bae, Yang Su (School of Applied Biosciences, Kyungpook National University)
Park, Eun-Hye (School of Applied Biosciences, Kyungpook National University)
Kim, Jeong-Sun (Agricultural Microbiology Division, National Academy of Agricultural Science, Rural Development Administration)
Choi, Jong-Yoon (Apple Research Station, National Institute of Horticultural & Herbal Science)
Lim, Seungmo (Biosystems and Bioengineering Program, University of Science and Technology (UST))
Moon, Jae Sun (Biosystems and Bioengineering Program, University of Science and Technology (UST))
Lee, Su-Heon (School of Applied Biosciences, Kyungpook National University)
Publication Information
Research in Plant Disease / v.20, no.4, 2014 , pp. 307-313 More about this Journal
Abstract
In May 2013, an angel's trumpet leaves showing mosaic and malformation symptoms were collected from Suwon city, Gyeonggi-do. An analysis of the collected sample by transmission electron microscopy observation showed filamentous rod particles of 720-800 nm in length. On the basis of the these observations, we performed PCR against three reported Potyviruses (Brugmansia mosaic virus, Colombian datura virus and Brugmansia suaveolens mottle virus), and the sample was positive for BruMV. Pathogenicity and host range test of BruMV was determined by mechanical inoculation. Solanaceae (tobacco, tomato and eggplant) and Amaranthaceae (ground cherry) appeared typical virus symptoms. To determine coat protein of this virus, we designed specific primer pairs, and performed PCR amplification, cloning, and sequencing. Phylogenetic analysis showed that BruMV-SW was most closely related to BruMV isolate SK. Comparison of the BruMV-SW coat protein nucleotide sequences showed 92% to 99% identities to the other BruMV isolates.
Keywords
Angel's trumpet; Bioassay; Brugmansia; Potyvirus;
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Times Cited By KSCI : 2  (Citation Analysis)
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1 Horne, R. W. and Wildy, P. 1963. Virus structure revealed by negative staining. Advan. Virus Res. 10: 101-170.
2 Bailey, L. H. and Bailey, E. Z. 1976. Hortus Third. A concise dictionary of plants cultivated in the United States and Canada. Pub, Co., Inc., New York, 1290 pp.
3 Bristol, M. L. 1969. Tree Datura drugs of the Colombian Sibunoy. Botanical Museum Leaflets, Harvard University. 22: 165-227.
4 Chellemi, D. O., Webster, C. G., Baker, C. A., Annamalai, M., Achor, D. and Adkins, S. 2011. Widespread occurrence and low genetic diversity of Colombian datura virus in Brugmansia suggest an anthropogenic role in virus selection and spread. Plant Dis. 95: 755-761.   DOI
5 Choi, S.-K., Cho, I.-S., Choi, G.-S. and Yoon, J.-Y. 2014. First report of Tomato spotted wilt virus in Brugmansia suaveolens in Korea. Plant Dis. 98: 1283.2.
6 Evans, W. C. and Lampard, J. F. 1972. Alkaloids of Datura suaveolens. Phytochemistry 11: 3293-3298.   DOI
7 Ilmberger, N., Willingmann, P., Adam, G. and Heinze, C. 2007. A Subgroup 1 Tobamovirus Isolated from Brugmansia sp. and its Detection by RT-PCR. J. Phytopathology 155: 326-332.   DOI
8 Lee, J. H., Park, S. J., Nam, M., Kim, M. J., Lee, J. B., Sohn, H., Choi, H. S., Kim, J.-S., Lee, J.-S., Moon, J.-S. and Lee, S.-H. 2010. Identification of a New Potyvirus, Keunjorong mosaic virus in Cynanchum wilfordii and C. auricilatum. Res. Plant Dis. 16: 238-246. (In Korean)   DOI
9 Lim, S., Zhao, F., Yoo, R. H., Igori, D., Lee, S. H., Lim, H. -S. and Moon, J. S. 2014. Characteristics of a Lettuce mosaic virus isolate infecting lettuce in Korea. Plant Pathology J. 30: 183-187.   과학기술학회마을   DOI
10 Lorenzi, H. and Souza, H. M. 2001. Plantas ornamentais do Brasil, 3rd ed. Nova Odessa, SP, Instituto Plantrum de Estudos da Flora Ltda. 1088 pp.
11 Zhao, F., Lim, S., Yoo, R. H., Lim, H.-S., Kwon, S.-Y., Lee, S.-H. and Moon, J. S. 2013. Complete genome sequence of a South Korean isolate of Brugmansia mosaic virus. Arch Virol. 158: 2019-2022.   DOI
12 Lucinda, N., Nagata, T., Inoue, -N. A. K., Salaroli, R. B. and Kitajima, E. W. 2008. Brugmansia suaveolens mottle virus, a novel potyvirus causing leaf mottling of Brugmansia suaveolens in Brazil. Arch Virol. 153: 1971-1976.   DOI
13 Nikolic, D., Stankovic, I., Vucurovic, A., Ristic, D., Milojevic, K., Bulajic, A. and Krstic, B. 2013. First Report of Tomato spotted wilt virus on Brugmansia sp. in Serbia. Plant Dis. 97: 850.1.
14 Salamon, P. and Palkovics, L. 2005. Occurrence of Colombian datura virus in Brugmansia hybrids, Physalis peruviana L. and Solanum muricatum Ait. in Hungary. Acta Virol. 49: 117-122.
15 Lucinda, N., Inoue, N.-A. K., Kitajima, E. W. and Nagata, T. 2010. Complete genome sequence of Brugmansia suaveolens mottle virus, a Potyvirus from an ornamental shrub. Arch Virol. 155: 1729-1732.   DOI