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http://dx.doi.org/10.5423/RPD.2014.20.1.037

Screening of Plant Extracts for Nemastatic Activity  

Kim, Donggeun (Organic Agriculture Research Institute)
Ryu, Younghyun (Organic Agriculture Research Institute)
Huh, Changseok (Organic Agriculture Research Institute)
Ryu, Junga (Organic Agriculture Research Institute)
Yeon, Ilkwon (Organic Agriculture Research Institute)
Lee, Younsu (Department of Applied Plant Science, Kangwon National University)
Publication Information
Research in Plant Disease / v.20, no.1, 2014 , pp. 37-49 More about this Journal
Abstract
A total of 2,714 methanol extracts of 1,677 plant species in 178 families were screened for nemastatic activity against Rhabditis sp. by 96-well microplate bioassay. The plant extracts with a concentration of $5,000{\mu}g/ml$ were mixed with aqueous nematode solution containing about 20 Rhabditis sp. and their activity was examined daily for 7 days. Out of 2,714 plant extracts examined in this test, 2,362 (87.0%) showed no negative influence on the nematode activity, while 187 (6.9%) inhibited nematode activity about 50%, 95 (3.5%) inhibited nematode activity over 90%, and 70 (2.6%) rather enhanced nematode activity. Among those showing over 50% nemastatic activity, 25 extracts were randomly selected for further screening with Rhabditis sp. and with juveniles of Meloidogyne incognita. The screening revealed that 11 extracts (44%) were consistently nemastatic. The 282 extracts with nemastatic activity could be of use in future studies on nematicidal plants.
Keywords
Meloidogyne sp. Nematicidal; Nemastatic; Plant extracts; Rhabditis;
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