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http://dx.doi.org/10.5423/RPD.2013.19.4.288

Production System of Virus-free Apple Plants Using Heat Treatment and Shoot Tip Culture  

Lee, Gunsup (Fruit Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration)
Kim, Jeong Hee (Apple Research Station, National Institute of Horticultural and Herbal Science, Rural Development Administration)
Kim, Hyun Ran (Future & Creation Strategy Team, Rural Development Administration)
Shin, Il Sheob (Fruit Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration)
Cho, Kang Hee (Fruit Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration)
Kim, Se Hee (Fruit Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration)
Shin, Juhee (Fruit Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration)
Kim, Dae Hyun (Fruit Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration)
Publication Information
Research in Plant Disease / v.19, no.4, 2013 , pp. 288-293 More about this Journal
Abstract
In worldwide, viral diseases of apple plants has caused the serious problems like reduced production and malformation of fruits. Also, the damages of apple plants by virus and/or viroid infection (Apple chlorotic leaf spot virus, Apple stem grooving virus, Apple mosaic virus, and Apple scar skin viroid) were reported in Korea. However there is few report about the protection approach against the infection by apple viruses. Therefore, this paper introduced the experimental protocol for the development of virus-free apple cultivars (Danhong, Hongan, Saenara, Summerdream). Apple plants were treated at $37^{\circ}C$ for 4 weeks and shoot tips were cultured in vitro. After heat treatment, the detection of apple viruses was performed by RT-PCR using virusspecific detection primers in new apple cultivars. With the heat treatments followed by in vitro shoot tip culture, the proportion of virus-free stocks of 'Danhong', 'Hongan', 'Saenara', and 'Summerdream' was 28%, 16%, 12%, and 12%, respectively. Taken together, this approach can be a good tool for production of virus-free apple stocks.
Keywords
Apple chlorotic leaf spot virus; Apple mosaic virus; Apple scar skin viroid; Apple stem grooving virus; Heat treatment;
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