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http://dx.doi.org/10.5423/RPD.2013.19.3.220

Development of RT-PCR and Nested PCR for Detecting Four Quarantine Plant Viruses Belonging to Nepovirus  

Lee, Siwon (Plant Quarantine Technology Center, Animal and Plant Quarantine Agency)
Kang, Eun-Ha (Plant Quarantine Technology Center, Animal and Plant Quarantine Agency)
Shin, Yong-Gil (Plant Quarantine Technology Center, Animal and Plant Quarantine Agency)
Lee, Su-Heon (School of Applied Biosciences, Kyungpook National University)
Publication Information
Research in Plant Disease / v.19, no.3, 2013 , pp. 220-225 More about this Journal
Abstract
For quarantine purpose, we developed the RT- and nested PCR module of Tomato black ring virus (TBRV), Arabis mosaic virus (ArMV), Cherry leafroll virus (CLRV) and Grapevine fanleaf virus (GFLV). The PCR modules, developed in this study make diagnosis more convenient and speedy because of same PCR condition. And also, the methods are more accurate because it can check whether the result is contamination or not using the mutation-positive control. We discard or return the 27 cases of Nepovirus infection seed by employing the module past 3 years. This study provides a rapid and useful method for detection of four quarantine plant viruses.
Keywords
Detection; Nepovirus; Nested PCR; Quarantine; RT-PCR;
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Times Cited By KSCI : 6  (Citation Analysis)
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