Browse > Article
http://dx.doi.org/10.5423/RPD.2013.19.2.095

Development of Efficient Screening Method for Resistance of Cabbage Cultivars to Black Rot Disease Caused by Xanthomonas campestris pv. campestris  

Lee, Ji Hyun (Research Center for Biobased Chemistry, Korea Research Institute of Chemical Technology)
Kim, Jin-Cheol (Research Center for Biobased Chemistry, Korea Research Institute of Chemical Technology)
Jang, Kyoung Soo (Research Center for Biobased Chemistry, Korea Research Institute of Chemical Technology)
Choi, Yong Ho (Research Center for Biobased Chemistry, Korea Research Institute of Chemical Technology)
Ahn, Kyoung Gu (Joeun Seeds)
Choi, Gyung Ja (Research Center for Biobased Chemistry, Korea Research Institute of Chemical Technology)
Publication Information
Research in Plant Disease / v.19, no.2, 2013 , pp. 95-101 More about this Journal
Abstract
Black rot caused by Xanthomonas campestris pv. campestris (Xcc) is one of the most serious diseases of crucifers world-wide. To establish the efficient screening method for resistant cabbage to Xcc, different inoculation methods, inoculation positions, growth stages of seedlings, and incubation temperatures after inoculation were investigated with the seven cabbage cultivars showing different resistance degrees to the pathogen. Clipping with mouse-tooth forceps was better inoculation method than piercing with 18 pins or cutting with scissors to distinguish the level of resistance and susceptibility. In inoculation using mouth-tooth forceps, clipping the edges of the leaves near veins is more effective than injuring the veins of the leaves directly. In addition, the inoculated plants kept at $22^{\circ}C$ showed more clear resistant and susceptible responses than those kept at 26 or $30^{\circ}C$. On the basis of the results, we suggest that an efficient screening method for resistance of cabbage cultivars to black rot is to clip the edges of the leaves near veins of the four-week-old seedlings with mouth-tooth forceps dipped in a suspension of Xcc at a concentration of $7{\times}10^7$ cfu/ml and incubate the inoculated plants in a growth room at $22^{\circ}C$ with 12-hr light a day.
Keywords
Black rot; Breeding; Crucifer crop; Disease resistance; Resistant screening;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
연도 인용수 순위
1 Marthe, F., Scholze, P., Griesbach, E. and Ryschka, U. 2002. New resistances to black rot (Xanthomonas campestris pv. campestris), clubroot (Plasmodiophora brassicae), blackleg and leaf spots (Phoma lingam) from genus Brassica to enhance resistance in cabbage (Brassica oleracea). 13. Crucifer Genetics Workshop, Davis, USA. 88 pp.
2 Staub, T. and Williams, P. H. 1972. Factors influencing black rot lesion development in resistant and susceptible cabbage. Phytopathology 62: 722-728.   DOI
3 Stewart, F. C. and Harding, H. A. 1903. Combating the black rot of cabbage by the removal of affected leaves. N. Y. Agric. Exp. St. Bull. 232: 43-65.
4 Sutton, M. D. and Williams, P. H. 1970. Relation of xylem plugging to black rot lesion development in cabbage. Can. J. Bot. 48: 391-401.   DOI
5 Taylor, J. D., Conway, J., Roberts, S. J., Astley, D. and Vicente, J. G. 2002. Sources and origin of resistance to Xanthomonas campestris pv. campestris in Brassica genomes. Phytopathology 92: 105-111.   DOI   ScienceOn
6 Tonguc, M., Earle, E. D. and Griffiths, P. D. 2003. Segregation distortion of Brassica carinata derived black rot resistance in Brassica oleracea. Euphytica 134: 269-276.   DOI
7 Vicente, J. G., Conway, J., Roberts, S. J. and Taylor, J. D. 2001. Identification and origin of Xanthomonas campestris pv. campestris races and related pathovars. Phytopathology 91: 492-499.   DOI   ScienceOn
8 Westman, A., Kresovich, S. and Dickson, M. H. 1999. Regional variation in Brassica nigra and other weedy crucifers for disease reaction to Alternaria brassicicola and Xanthomonas campestris pv. campestris. Euphytica 106: 253-259.   DOI   ScienceOn
9 Williams, P. H., Staub, T. and Sutton, J. C. 1972. Inheritance of resistance in cabbage to black rot. Phytopathology 62: 247-252.   DOI
10 Williams, P. H. 1980. Black rot: A continuing threat to world crucifers. Plant Dis. 64: 736-742.   DOI
11 Smith, E. F. 1911. Bacteria in Relation to Plant Diseases; History, General Considerations, Vascular Diseases. Vol. II. Carnegie Inst., Washington D. C. 262 pp.
12 Jo, S. J., Shim, S. A., Jang, K. S., Choi, Y. H., Kim, J. C. and Choi, G. J. 2012. Development of efficient screening method for resistant cabbage and broccoli to Plasmodiophora brassicae. Res. Plant Dis. 18: 86-92. (In Korean)   과학기술학회마을   DOI   ScienceOn
13 Kamoun, S., Kadmar, H. V., Tola, E. and Kado, C. I. 1992. Incompatible interaction between crucifers and Xanthomonas campestris involve a vascular hypersensitive response: Role of the hrpX locus. Mol. Plant-Microbe Interact. 5: 22-33.   DOI
14 Kocks, C. G., Ruissen, M. A., Zadoks, J. C. and Duijkers, M. G. 1998. Survival and extension of Xanthomonas campestris pv. campestris in soil. Eur. J. Plant Pathol. 104: 911-923.   DOI   ScienceOn
15 Lee, S. O. 2007. Agricultural products story (21) Cabbage. Agrochemical News Magazine 28: 48. (In Korean)
16 Monakhos, G. F. and Djalilov, F. S. 2000. Genetic sources and methods for estimation of brassicas resistance to black rot. J. Russ. Phytopathol. Soc. 1: 83-88.
17 Sharma, B. R., Swarup, V. and Chatterjee, S. S. 1972. Inheritance of resistance to black rot in cauliflower. Can. J. Genet. Cytol. 14: 363-370.   DOI
18 Oh, I. N., Choi, S. H., Park, S. Y., Lim, Y. P. and An, G. H. 2011. Effect of season, tissue position and color on content of amino acids in cabbage (Brassica oleracea). CNU J. Agr. Sci. 38: 79-86. (In Korean)   과학기술학회마을
19 Quazi, M. H. 1988. Interspecific hybrids between Brassica napus and Brassica oleracea L. developed by embryo culture. Theor. Appl. Genet. 75: 309-318.   DOI
20 Schaad, N. W. and Dianese, J. C. 1981. Cruciferous weeds as sources of inoculum of Xanthomonas campestris in black rot of crucifers. Phytopathology 71: 1215-1220.   DOI
21 Slusarenko, A. J., Fraser, R. S. S. and van Loon, L. C. 2000. Mechanisms of resistance to plant diseases. In: Black Rot of Crucifers, ed. by A. M. Alvarez, pp. 21-52. Kluwer Academic Publishers, Netherlands.
22 Bain, D. C. 1952. Reaction of Brassica seedlings to black rot. Phytopathology 42: 497-500.
23 Bain, D. C. 1955. Resistance of cabbage to black rot. Phytopathology 45: 35-37.
24 Cartea, M. E., Velasco, P., Obregon, S., Padilla, G. and Haro, A. D. 2008. Seasonal variation in glucosinolate content in Brassica oleracea crops grown in northwestern Spain. Phytochemistry 69: 403-410.   DOI   ScienceOn
25 Dickson, M. H. and Hunter, J. E. 1987. Inheritance of resistance in cabbage seedlings to black rot. Hort. Sci. 22: 108-109.
26 Hunter, J. E., Dickson, M. H. and Ludwig, J. 1987. Source of resistance to black rot of cabbage expressed in seedlings and adult plants. Plant Dis. 71: 263-266.   DOI
27 Eum, H. L., Lee, Y. H., Hong, S. J., Shin, I. S. and Yeoung, Y. R. 2012. Quality change during harvest time and storage of various cabbages grown on high land by different transplanting times. J. Bio-Environ. Control 21: 95-101.
28 Griesbach, E., Loptien, H. and Miersch, U. 2003. Resistance to Xanthomonas campestris pv. campestris (Pammel) Dowson in cabbage Brassica oleracea L. J. Plant Dis. Prot. 110: 461-475.   DOI
29 Guo, H., Dickson, M. H. and Hunter, J. E. 1991. Brassica napus sources of resistance to black rot of crucifers and inheritance of resistance. Hort. Sci. 26: 1545-1547.
30 Ignatov, A., Kuginuki, Y. and Hida, K. 1998. Race-specific reaction of resistance to black rot in Brassica oleracea. Eur. J. Plant Pathol. 104: 821-827.   DOI   ScienceOn
31 Jensen, B. D., Massomo, S. M. S., Swai, I. S., Hockenhull, J. and Andersen, S. B. 2005. Field evaluation for resistance to the black rot pathogen Xanthomonas campestris pv. campestris in cabbage (Brassica oleracea). Eur. J. Plant Pathol. 113: 297-308.   DOI