Browse > Article
http://dx.doi.org/10.5423/RPD.2012.18.4.354

Practical Application of Dioscorea quinqueloba Extract for the Control of Citrus Green Mold  

Lee, Ji Hyun (Department of Applied Biology, Chungnam National University)
Kang, Sung Woo (Department of Applied Biology, Chungnam National University)
Song, Jeong Young (Department of Applied Biology, Chungnam National University)
Kim, Hong Gi (Department of Applied Biology, Chungnam National University)
Publication Information
Research in Plant Disease / v.18, no.4, 2012 , pp. 354-360 More about this Journal
Abstract
This study tested the antifungal compound obtained from a medicinal plant, Dioscorea quinqueloba Thunb., in order to search the possibility of practical application of this product in agriculture through evaluating its activity using the citrus fruits. The extract of D. quinqueloba Thunb., which has the strongest antifungal activity, was selected as a candidate among 101 plant extracts. Based on this examination concerning antifungal activity of the product on Penicillium digitatum in vitro, it was confirmed its effect of mycelial growth inhibition showed over 87% at 0.5 mg/ml concentration. This natural product showed the stability of the substance, as it was not significantly influenced by pH, temperature, or ultraviolet radiation. While citrus fruits were stored at room temperature, P. digitatum was inoculated into them in order to prepare a similar environmental conditions with epidemic occurrence of the mold. As the result of our investigation, the disease preventive effects of the active antifungal substance evidenced a 100% at 0.5 mg/ml. When the phytotoxicity of the selected natural product on citrus at 2 mg/ml was assessed, we noted no toxic effects. Based on the superior preventive effects from this natural product extracted from the plant, it is presumed to be very useful in agricultural applications for the control of green mold, P. digitatum, which has been occurred often the biggest problem in the storage of citrus fruits.
Keywords
Biological control; Dioscorea quinqueloba Thunb.; Penicillium digitatum; Plant extract; Storage disease;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
연도 인용수 순위
1 Porat, R., Vinocur, V., Weiss, B., Cohen, L. and Droby, S. 1999b. Effects of various elicitors on the resistance of citrus fruit against pathogens. Phytoparasitica 27: 157-158.
2 Porat, R., Pavoncello, D., Peretz, J., Weiss, B., Daus, A., Cohen, L., Ben-Yehoshua, S., Droby, S. and Lurie, S. 2000. Induction of resistance to Penicillium digitatum and chilling injury in Star Ruby grapefruit by a short hot water rinse and brushing treatment. J. Hort. Sci. Biotechnol. 75: 428-432.
3 Sautour, M., Mitaine-Offer, A. C. and Lacaille-Dubois, M. A. 2007. The Dioscorea genus: a review of bioactive steroid saponins. J. Nat. Med. 61: 91-101.   DOI
4 Smilanick, J. L., Mansour, M. F., Mlikota Gabler, F. and Goodwine, W. R. 2006. The effectiveness of pyrimethanil to inhibit germination of Penicillium digitatum and to control citrus green mold after harvest. Postharvest Biol. Technol. 42: 75-85.   DOI
5 Tzortzakis, N. G. and Economakis, C. D. 2007. Antifungal activity of lemongrass (Cympopogon citratus L.) essential oil against key postharvest pathogens. Innov. Food Sci. Emerg. Technol. 8: 253-258.   DOI
6 Yahyazadeh, M., Omidbaigi, R., Zare, R. and Taheri, H. 2008. Effect of some essential oils on mycelial growth of Penicillium digitatum Sacc. World J. Microbiol. Biotechnol. 24: 1445-1450.   DOI
7 Al-Mughrabi, K. 2003. Antimicrobial activity of extracts from leaves, stems and flowers of Euphorbia macroclada against plant pathogenic fungi. Phytopathol. Mediterr. 42: 245-250.
8 Brown, G. E. and Eckert, J. W. 2000. Penicillium Decays. In compendium of Citrus Diseases 2nd. Timmer, L. W., Garnsey, S. M. and Graham, J. H., eds. The American Phytopathological Society, St. Paul, Mn. 41-42.
9 Droby, S., Chalutz, E., Horev, B., Cohen, L., Gaba, V., Wilson, C. and Wisniewski, M. 1993. Factors affecting UV-induced resistance in grapefruit against the green mould decay caused by Penicillium digitatum. Plant Pathol. 42: 418-424.   DOI
10 Costa, E., Teixido, N., Usall, J., Atares, E. and Vinas, I. 2001. Production of the biocontrol agent Pantoea agglomerans strain CPA-2 using commercial products and by-products. Appl. Microbiol. Biotechnol. 56: 367-371.   DOI
11 Droby, S., Porat, R., Cohen, L., Weiss, B., Shapira, B., Philosoph-Hadas, S. and Meir, S. 1999. Suppressing green mold decay in grape fruit with postharvest jasmonates application. J. Am. Soc. Hort. Sci. 124: 184-188.
12 Fogliata, G. M., Torres, L. G. J. and Ploper, L. D. 2001. Detection of imazalil-resistant strains of Penicillium digitatum Sacc. In citrus packing houses of Tacuman Province (Argentina) and their behaviour against current employed and alternative fungicides. Rev. Ind. Agric. Tacuman. 77: 71-75.
13 Hao, W., Li, H., Hu, M., Yang, L. and Rizwan-ul-Haq, M. 2011. Integrated control of citrus green and blue mold and sour rot by Bacillus amyloliquefaciens in combination with tea saponin. Postharvest Biol. Technol. 59: 316-323.   DOI
14 Holmes, G. J. and Eckert, J. W. 1999. Sensitivity of Penicillium digitatum and P. italicum to postharvest citrus fungicides in California. J. Phytopathol. 89: 716-721.   DOI
15 Janisiewicz, W. J. and Korsten, L. 2002. Biological control of postharvest diseases of fruits. Annu. Rev. Phytopathol. 40: 411-441.   DOI   ScienceOn
16 Kim, J. B., Kim, N. K., Lim, J. H., Kim, S. I., Kim, H. H., Song, J. Y. and Kim, H. G. 2009. Environment friendly control of gray mold, a ginseng storage disease using essential oils. Res. Plant Dis. 15: 236-241.   과학기술학회마을   DOI
17 Mekbib, S. B., Regnier, T. J. and Korsten, L. 2007. Control of Penicillium digitatum on citrus fruit using two plant extracts and study of their mode of action. Phytoparasitica 35: 264-276.   DOI
18 Kinay, P., Mansour, M. F., Gabler, F. M., Margosan, D. A. and Smilanick, J. L. 2007. Characterization of fungicide-resistant isolates of Penicillium digitatum collected in California. Crop Prot. 26: 647-656.   DOI
19 Kum, E. J., Park, S. J., Lee, B. H., Kim, J. S., Son, K. H. and Sohn, H. Y. 2006. Antifungal activity of phenanthrene derivatives from aerial bulbils of Dioscorea batatas Decne. J. Life Sci. 16: 647-652.   과학기술학회마을   DOI
20 Larrigaudiere, C., Pons, J., Torres, R. and Usall, J. 2002. Storage performance of clementines treated with hot water, sodium carbonate and sodium bicarbonate dips. J. Hort. Sci. Biotechnol. 77: 314-319.
21 Paik, S. B. and Chung, I. M. 1997. Effect of medicinal plant extracts on apple storage diseases. Korean J. Plant Pathol. 13: 57-62.   과학기술학회마을
22 Palou, L., Smilanick, J. L., Usall, J. and Vinas, I. 2001. Control of postharvest blue and green molds of oranges by hot water, sodium carbonate, and sodium bicarbonate. Plant Dis. 85: 371-376.   DOI
23 Paster, N. and Bullerman, L. B. 1988. Mould spoilage and mycotoxin formation in grains as controlled by physical means. Int. J. Food Microbiol. 7: 257-265.   DOI
24 Porat, R., Lers, A. S., Dori, L., Cohen, B., Weiss, A., Daus, C. S., Wilson, S. and Droby, S. 1999a. Induction of chitinase and ${\beta}$-1,3-endoglucanase proteins by UV irradiation and wounding in grapefruit peel tissue. Phytoparasitica 27: 233-238.   DOI