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http://dx.doi.org/10.5423/RPD.2012.18.4.290

Evaluation of Resistance in Pepper Germplasm to Cucumber mosaic virus by High Resolution Melting Analysis  

Ro, Na Young (National Agrobiodiversity Center, National Academy of Agricultural Science, Rural Development Administration)
Hur, On Sook (National Agrobiodiversity Center, National Academy of Agricultural Science, Rural Development Administration)
Ko, Ho Cheol (National Agrobiodiversity Center, National Academy of Agricultural Science, Rural Development Administration)
Kim, Sang Gyu (National Agrobiodiversity Center, National Academy of Agricultural Science, Rural Development Administration)
Rhee, Ju Hee (National Agrobiodiversity Center, National Academy of Agricultural Science, Rural Development Administration)
Gwag, Jae-Gyun (National Agrobiodiversity Center, National Academy of Agricultural Science, Rural Development Administration)
Kwon, Jin-Kyung (Department of Plant Science, and Plant Genomics and Breeding Institute, College of Agriculture and Life Sciences, Seoul National University)
Kang, Byoung-Cheorl (Department of Plant Science, and Plant Genomics and Breeding Institute, College of Agriculture and Life Sciences, Seoul National University)
Publication Information
Research in Plant Disease / v.18, no.4, 2012 , pp. 290-297 More about this Journal
Abstract
In this study, total number of 1941 Capsicum accessions conserved at RDA Genebank was evaluated for their response to Cucumber mosaic virus (CMV). These accessions were composed with 9 species originated from 89 countries, included 839 Capsicum annuum, 277 C. baccatum, 395 C. chinense, 343 C. frutescens, 49 C. pubescens, and other 38 wild pepper species (C. chacoense, C. galapagoense, etc.). Resistant to CMV was screened with the 240H02SP6 SNP marker related to the Cmr1 (Cucumber mosaic resistance 1). Eighty nine accessions of pepper germplasm were resistant to CMV based on the marker. One hundred sixty two accessions showed heterozygosity. One thousand two hundred seventy accessions were susceptible to CMV. Four hundred twenty accessions did not show distinction by 240H02SP6 marker. These 89 resistant pepper germplasm can be used in a pepper breeding program against CMV.
Keywords
Capsicum; Cucumber mosaic virus; Pepper; Resistant resources;
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1 Abdalla, O. A., Desjardine, P. R. and Dodds, J. A. 1991. Identification, disease incidence, and distribution of viruses infecting peppers in California. Plant Dis. 75: 1019-1023.   DOI
2 Benner, C. P., Kuhn, C. W., Demski, J. W., Dobson, J. W., Colditz, P. and Nutter, F. W. Jr. 1985. Identification and incidence of pepper viruses in northeastern Georgia. Plant Dis. 69: 999-1001.
3 Boccardo, G. and Conti, M. 1973. Purification and properties of nasturtium ringspot virus. J. Phytopathol. 78: 14-24.   DOI
4 Cai, W. Q., Fang, R. X., Shang, H. S., Wang, X., Zhang, F. L., Li, Y. R., Zhang, J. C., Cheng, X. Y., Wang, G. L. and Mang, K. Q. 2003. Development of CMV- and TMV-resistant transgenic chili pepper: Field performance and biosafety assessment. Mol. Breeding 11: 25-35.   DOI   ScienceOn
5 Chagne, D., Gasic, K., Crowhurst, R. N., Han, Y., Bassett, H. C., Bowatte, D. R., Lawrence, T. J., Rikkerink, E. H., Gardiner, S. E. and Korban, S. S. 2008. Development of a set of SNP markers present in expressed genes of the apple. Genomics 92: 353-358.   DOI   ScienceOn
6 Cho, D. W., Oh, J. P., Park, K. W. and Chung, K. H. 2009. Analysis of agricultural characteristics for the virus resistant GM pepper plants grown in three different regions in Korea. Korean J. Hort. Sci. Technol. 27: 521-529.
7 Cho, J. D., Lee, S. H., Kim, J. S., Choi, K. S. and Chung, B. N. 2007. Study of occurrence of virus disease on the pepper in Korea. BioWave 9: 1-8. (In Korean)
8 Croxford, A. E., Rogers, T., Caligari, P. D. S. and Wilkinson, M. J. 2008. High-resolution melt analysis to identify and map sequence-tagged site anchor points onto linkage maps: a white lupin (Lupinus albus) map as an exemplar. New Phytol. 180: 594-607.   DOI
9 Florini, D. A. and Zitter, T. A. 1987. Cucumber mosaic virus (CMV) in pepper (Capsicum annuum L.) in New York and associated yield losses. Phytopathology 77: 652. (Abstract)
10 Gracia, O. and Gutierrez, L. S. 1982. Broad bean wilt virus in pepper crops in Argentina. Phytopath. Medit. 21: 107-109.
11 Hofinger, B. J., Jing, H. C., Hammond-Kosack, K. E. and Kanyuka, K. 2009. High-resolution melting analysis of cDNA-derived PCR amplicons for rapid and cost-effective identification of novel alleles in barley. Theor. Appl. Genet. 119: 851-865.   DOI
12 Imoto, M. 1975. Studies on mosaic disease of sweet pepper (Capsicum frutescens L.) 2. Occurrence of mosaic disease of sweet pepper and kinds of its causal viruses in Hiroshima prefecture. Bull. Hiroshima Agr. Expt. Station, Japan 36: 56-66.
13 Kim, D. H., Cho, J. D., Kim, J. H., Kim, J. S. and Cho, E. K. 2005. Ultrastructural characteristics of necrosis and stunt disease in red pepper by the mixed infections of Tobacco mosaic virus-U1 and Pepper mild mottle virus and Pepper mottle virus. Plant Pathology J. 21: 252-257.   DOI
14 Jeong, H. J., Kwon, J. K., Pandeya, D., Hwang, J. N., Hoang, N. H., Bae, J. H. and Kang, B. C. 2012. A survey of natural and ethyl methane sulfonate-induced variations of eIF4E using high-resolution melting analysis in Capsicum. Mol. Breeding 29: 349-360.   DOI
15 Joshi, R. D. and Dubey, L. N. 1973. Assessment of losses due to CMV on chili. Science and Culture 39: 521-522.
16 Kang, W. H., Hoang, N. H., Yang, H. B., Kwon, J. K., Jo, S. H., Seo, J. K., Kim, K. H., Choi, D. and Kang, B. C. 2010. Molecular mapping and characterization of a single dominant gene controlling CMV resistance in peppers (Capsicum annuum L.). Theor. Appl. Genet. 120: 1587-1596.   DOI
17 Kim, S. H., Yoon, J. B., Do, J. W. and Park, H. G. 2008. A major recessive gene associated with anthracnose resistance to Colletotrichum capsici in chili pepper (Capsicum annuum L.). Breed. Sci. 58: 137-141.   DOI
18 Laird, E. F., Desjardins, P. R. and Dickson, R. C. 1964. Tobacco etch virus and potato virus Y from pepper in southern California. Plant Dis. Rep. 48: 772-776.
19 Lee, S. H., Lee, J. B., Kim, S. M., Choi, H. S., Park, J. W., Lee, J. S., Lee, K. W. and Moon, J. S. 2004. The incidence and distribution of viral diseases in pepper by cultivation types. Res. Plant Dis. 10: 231-240. (In Korean)   DOI
20 Lee, Y. H., Jung, M., Shin, S. H., Lee, J. H., Choi, S. H., Her, N. H., Lee, J. H., Ryu, K. H., Paek, K. Y. and Harn, C. H. 2009. Transgenic peppers that are highly tolerant to a new CMV pathotype. Plant Cell Rep. 28: 223-232.   DOI   ScienceOn
21 Palukaitis, P., Roossinck, M. J., Dietzgen, R. G. and Francki, R. I. B. 1992. Cucumber mosaic virus. Adv. Virus Res. 41: 281-341.   DOI
22 Lehmensiek, A., Sutherland, M. W. and McNamara, R. B. 2008. The use of high resolution melting (HRM) to map single nucleotide polymorphism markers linked to a covered smut resistance gene in barley. Theor. Appl. Genet. 117: 721-728.   DOI   ScienceOn
23 Makkouk, K. M. and Gumpf, D. J. 1976. Characterization of potato virus Y strains isolated from pepper. Phytopathology 66: 576-581.   DOI
24 Pae, D. H., Chae, Y., Wang, T. C., Engle, L. M. and Shanmugasundaram, S. 2005. Selection of new breeding materials with resistance to anthracnose in Capsicum annuum. J. Korean Soc. Hort. Sci. 46: 10-12.
25 Park, S. W., An, S. J., Yang, H. B., Kwon, J. K. and Kang, B. C. 2009. Optimization of high resolution melting analysis and discovery of single nucleotide polymorphism in Capsicum. Hort. Environ. Biotechnol. 50: 31-39.   과학기술학회마을
26 Purcifull, D. E., Zitter, T. A. and Hiebert, E. 1975. Morphology, host range and serological relationships of pepper mottle virus. Phytopathology 65: 559-562.   DOI
27 Wittwer, C. T., Reed, G. H., Gundry, C. N., Vandersteen, J. G. and Pryor, R. J. 2003. High-resolution genotyping by amplicon melting analysis using LCGreen. Clin. Chem. 49: 853-860.   DOI   ScienceOn
28 Yankulova, M. and Kaitazova, P. 1979. Broad bean wilt virus-a new virus on pepper in Bulgaria. Grradinarska I Lozarska Nanka. 16: 48-57.
29 Yoon, J. B., Do, J. W., Yang, D. C. and Park, H. G. 2004. Interspecific cross compatibility among five domesticated species of Capsicum genus. J. Korean Soc. Hort. Sci. 45: 324- 329.   과학기술학회마을