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http://dx.doi.org/10.5423/RPD.2011.17.3.302

Suppression Effect and Mechanism of Citrus Scab in the Citrus Pre-inoculated with Rhizobacterial Strains  

Kim, So-Yeon (Plant Resources and Environment, College of Applied Life Sciences, The Research Institute for Subtropical Agriculture and Biotechnology, Jeju National University)
Hyun, Jae-Wook (Citrus Research Station, National Institute of Horticultural and Herbal Science, Rural Development Administration)
Jeun, Yong-Chull (Plant Resources and Environment, College of Applied Life Sciences, The Research Institute for Subtropical Agriculture and Biotechnology, Jeju National University)
Publication Information
Research in Plant Disease / v.17, no.3, 2011 , pp. 302-310 More about this Journal
Abstract
Elsinoe fawcettii is one of major pathogenic fungi which cause citrus scab diseases, resulting in fruit blemishes that reduce the economic value of fruit. By increasing interest to safe products of crops, the alternative methods of disease control is highly required. We investigated whether the 215 bacterial strains isolated from Jeju Island possess antifungal effect or suppression effect on the symptom development by Elsinoe fawcettii on citrus. Among them, three bacterial strains THJ 609-3, MRL408-3, and TRH423-3 that exhibited antifungal capacity against Elsinoe fawcettii were selected. To illustrate the disease suppression mechanism, pre-inoculation with the selected bacterial strains was carried out whether could suppress the citrus crab on the leaves. The observation with a fluorescence microscope revealed that the selected bacteria could decrease the number of fungal spores. The ratio of germ tube formation was also decreased by the selected bacterial strains at one day after fungus challenge. The strain THJ 609-3 was identified as Pseudomonas putida as a result of analyzing the internal transcript spaces of the rhizobacterial rDNA. The strains MRL 408-3 and TRH 423-3 were identified as Burkholderia gladioli. Our results may be valuable when the selected rhizobacterial strains used as the environment-friendly microbe for biological control on citrus scab caused by Elsinoe fawcettii.
Keywords
Antifungal activity; Biological control; Fluorescent microscopy;
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1 Thomashow, L. S. and Weller, D. M. 1996. Current concepts in the use of introduced bacteria for biological disease control: mechanisms and antifungal metabolites. In: Plant-microbe interactions, vol. 1., ed. by G. Stacey and N.T. Keen, Campman and Hall, pp. 187-235, New York, N. Y.
2 Timmer, L. W., Priest, M., Broadbent, P. and Tan, M. K. 1996. Morphological and pathological characterization of species of Elsinoe causing scab diseases of citrus. Phytopathology 86: 1032-1038.   DOI
3 Timmer, L. W. 2000. Scab diseases. In: Compendium of Citrus Diseases. 2nd ed., ed. by L. W. Timmer, S. M. Garnsey, and J. H. Graham, American Phytopathological Socienty Press. St. Paul. M N. pp. 31-32.
4 Kong, H. G., Chun, O. J., Choi, K. H., Lee, K. Y., Baek, J. W., Kim, H. J., Senthilkumar, M., Moon, B. J. and Lee, S. W. 2010. Formulation of Bacillus amyloliquefaciens A-2 and its efficacy to control tomato leaf mold caused by Fulvia fulva. Res. Plant Dis. 16: 27-34. (In Korean)   과학기술학회마을   DOI
5 Lee, C. S., Kim, K. D., Hyun, J. W. and Jeun, Y. C. 2003. Isolation of rhizobacteria in Jeju island showing anti-fungal effect against fungal plant pathogens. Mycobiology 31: 251-254.   과학기술학회마을   DOI
6 Lee, K. H. and Jeun, Y. C. 2005. Observations of infection structures after inoculation with Colletotrichum orbiculare on the leaves of cucumber plants pre-inoculated with two tacterial strains Pseudomonas putida or Micrococcus luteus. Korean Soc. Mycol. 33: 131-136.   과학기술학회마을   DOI
7 Nam, M. H., Choi, J. P., Kim, H. J., Lee, J. J., Lim, K. H., Kim, Y. G., Kim, H. T. and Jeun, Y. C. 2010. Controlling activity of Bacillus subtilis KB-401 against cucumber powdery mildew caused by Sphaerotheca fusca. Korean. J. Pesticide Sci. 14: 49-53. (In Korean)   과학기술학회마을
8 Raaijmakers, J. M., Weller, D. M. and Thomashow, L. S. 1997. Frequency of antibiotic-producing Pseudomonas spp. in natural environments. Appl. Environ. Microbiol. 63: 881-887.
9 An, Y. K., Kang, S. C., Kim, K. D., Hwang, B. K. and Jeun, Y. C. 2010.12. Enhanced defense responses of tomato plants against late blight pathogen Phytophthora infestans by pre-inoculation with rhizobacteria. Crop prot. 29: 1406-1412.   DOI   ScienceOn
10 Weller, D. M. 1988. Biological control of soilborn plant pathogens in the rhizosphere with bacteria. Annu. Rev. Pathol. 13: 129-131.
11 Yang. C., Dong, M., Yuan Y., Huang, Y., Guo, X. and Qiao, C. 2007. Reductive transformation of parathion and methyl parathion by Bacillus sp. Biotechnol. Lett. 29: 487-493.   DOI
12 Kang, S. J., Kim, J. H. and Joo, G. J. 2005. Isolation of antagonistic bacteria against Fusarium oxysporum and physicochemical properties of compost mixed with microbial formulation. Korean. J. Hort. Sci. Technol. 23: 342-350. (In Korean)   과학기술학회마을
13 Kim, J. H., Choi, Y. H., Kang, S. J., Rhee, I. K. and Joo, G. J. 2002. Biocontrol of vegetables damping-off by Bacillus ehimensis YJ-37. Korean. J. Life Sci. 12: 416-422. (In Korean)   과학기술학회마을   DOI
14 Hyun, J. W., Timmer, L. W., Lee, S. C., Yun, S. H., Ko, S. W. and Kim, K. S. 2001. Pathological characterization and molecular analysis of Elsinoe isolates causing scab diseases of citrus in Jeju isolates in Korea. Plant Dis. 85: 1013-1017.   DOI
15 Cho, J. I., Cho, J. Y., Park, Y. S., Yang, S. Y. and Heo, B. G. 2007. Selection and identification of Actinomyces, and its biological control effect against the bacterial blossom blight infected in kiwifruits. Korean. J. Sci. Technol. 25: 235-240. (In Korean)   과학기술학회마을
16 David, M. W. 1988. Biological control of soilborne plant pathogens in the rhizosphere with bacteria. Annu. Rev. Phytopathil. 26: 379-407.   DOI
17 Hong, S. H. and Cho, K. S. 2007. Effect of Plant, rhizobacteria and physicocheical factors on the phytoremediation of contaminated soil. Korean. J. Microbiol. 35: 261-271. (In Korean)   과학기술학회마을
18 Jeun, Y. C., Siegrist, J. and Buchenauer. H. 2000. Biochemical and cytological studies onmechanisms of systemically incuced resistance in tomato plants against Phytophthora infestans. J. Phytopathol. 148: 129-140.
19 Jeun, Y. C., Lee, Y. J. and Bae, Y. S. 2004. Rhizobacteriamediated incuced systemic resistance in cucmber plants against anthracnose disease caused by Colletotrichum orbiculare. Korean. J. Plant. Pathol. 20: 172-176. (In Korean)   과학기술학회마을   DOI