Browse > Article
http://dx.doi.org/10.5423/RPD.2011.17.2.211

Characterization of an Isolate of Cucumber mosaic virus from Raphanus sativus L.  

Rhee, Sun-Ju (Department of Integrative Plant Sciences, Chung-Ang University)
Hong, Jin-Sung (Institute of Natural Sciences, Seoul Women's University)
Choi, Jang-Kyung (Department of Applied Biology, Kangwon National University)
Kim, Eun-Ji (Department of Integrative Plant Sciences, Chung-Ang University)
Lee, Gung-Pyo (Department of Integrative Plant Sciences, Chung-Ang University)
Publication Information
Research in Plant Disease / v.17, no.2, 2011 , pp. 211-215 More about this Journal
Abstract
Cucumber mosaic virus (CMV)-like isolate was collected from Raphanus sativus (cv. Choon-hyang), which showed mosaic symptoms. The isolate was confirmed to a strain of CMV by host responses in Vigna unguiculata, Chenopodium amaranticolor and Gomphrena globosa, by viral genome composition with RT-PCR and PCR-RFLP, and by serological analysis. Symptom developed by the strain of CMV was severe in Nicotiana benthamiana, N. glutinosa, N. tabacum (cv. Samsun, cv. Xanthi), Cucumis melo (cv. Early hanover), Cucumis sativus (cv. White wonder), Capsicum annuum (cv. Chung-yang and cv. Geum-top), but mild symptom was developed in Raphanus sativus (cv. Choon-hyang), Brassica rapa ssp. pekinensis (cv. Bul-Am No. 3), and B. juncea (cv. Daenong Jukgot). Newly isolated strain of CMV could infect diverse crops including Solanaceae, Cucurbitaceae and Brassicaceae. We designated the new strain of CMV as Gn-CMV based on the novel infectivity of Brassicaceae. In double-stranded (ds) RNA analysis, Gn-CMV consisted of 3.3, 3.0, and 2.2 kb genomes likewise other strains of CMV. SDS-polyacrylamide gel electrophoresis (SDS-PAGE) showed 28 kDa of the CMV coat protein. By restriction enzyme mapping using Cac8I, ClaI and MspI of RT-PCR products indicated that Gn-CMV belongs to CMV subgroup I.
Keywords
dsRNA; Host range analysis; PCR-RFLP; RT-PCR; SDS-PAGE;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Wang, H., Culver, J. N. and Stubbs, G. R. T. 1997. Structure of Ribgrass mosaic virus at 2,9 A resolution.: evolution and taxonomy of tobamoviruses. J. Mol. Biol. 269: 769-779.   DOI
2 Yamanaka, T., Komatan, H., Meshi, T., Naito, S., Ishikawa, M. and Ohno, T. 1998. Complete nucleotide sequence of the genomic RNA of Tobacco mosaic virus strain Cg. Virus Genes 16: 173-176.   DOI
3 Aguilar, I., Sanchez, F., Martin-Martin, A., Martinez-Herrera, D. and Ponz, F. 1996. Nucleotide sequence of Chinese rape mosaic virus (oilseed rape mosaic virus), a crucifer tobamovirus infectious on Arabidopsis thaliana. Plant Mol. Biol. 30: 191-197.   DOI
4 Chen, Y., Chen, J., Zhang, H., Tang, X. and Du, Z. 2007. Molecular evidence and sequence analysis of a natural reassortant between Cucumber mosaic virus subgroup IA and II strains. Virus Genes 35: 405-413.   DOI
5 Choi, J. K., Kim, H. J., Hong, J. S., Kim, D. W. and Lee, S. Y. 1998. Identification and differentiation of Cucumber mosaic virus isolates in Korea. Korean J. Plant Pathol. 14: 7-12.
6 Choi, J. K., Sung, M. Y., Jung, H. J., Hong, J. S. and Lee, S. Y. 2001. Cross protection effectiveness of Cucumber mosaic virus (CMV) isolates associated with satellite RNA for prevention of CMV disease in pepper plants. Res. Plant Dis. 7: 155-163.   과학기술학회마을
7 Dodds, J. A. 1993. DsRNA in diagnosis. In: Diagnosis of Plant Virus Diseases, ed. by R.E.F. Matthews. pp. 273-295. CRC Press, Boca Raton, USA.
8 Dorokhov, Y. L., Ivanov, P. A., Novikov, V. K., Agranovsky, A. A., Morozov, S. Y., Efimov, V. A., Casper, R. and Atabekov, J. G. 1994. Complete nucleotide sequence and genome organization of a tobamovirus infecting cruciferae plants. FEBS Lett. 350: 5-8.   DOI
9 Gould, A. R. and Symons, R. H. 1978. Alfalfa mosaic virus RNA. Determination of the sequence homology between the four RNA species and a comparison with the four RNA species of Cucumber mosaic virus. Eur. J. Biochem. 4: 3787-3802.
10 Lartey, R. T., Hartson, S. D., Pennington, R. E., Sherwood, J. L. and Melcher, U. 1993. Occurrence of a vein-clearing tobamovirus in turnip. Plant Dis. 77: 21-24.   DOI
11 Morris, T. J. and Dodds, J. A. 1979. Isolation and analysis of double-stranded RNA from virus-infected plant and fungal sissue. Phytopathology 69: 854-858.   DOI
12 Tomlinson, J. A. 1987. Epidemiology and control of virus diseases of vegetables. Ann. Appl. Biol. 110: 661-681.   DOI
13 Owen, J. and Palukaitis, P. 1988. Characterization of Cucumber mosaic virus. I. Molecular heterogenecity mapping of RNA3 in eight CMV strains. Virology 166: 496-502.
14 Palukaitis, P., Roossinck, M. J., Dietzgen, R. G. and Francki, R. I. B. 1992. Cucumber mosaic virus. Adv. Virus. Res. 41: 281- 348.   DOI
15 Roossinck, M. J., Zhang, L. and Hellwald, K. H. 1999. Rearrangement in the 5' nontranslated region and phylogenetic analysis of Cucumber mosaic virus RNA3 indicated radialevolution of three subgroups. J. Virol. 73: 6752-6758.
16 Tomlinson, J. A., Carter, A. L., Dale, W. T. and Simpsom, C. J. 1970. Weed plants as sources of cucumber mosaic virus. Ann. Appl. Biol. 66: 11-16.   DOI