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http://dx.doi.org/10.5423/RPD.2010.16.1.035

Suppressive Effect of Bacterial Isolates from Plant Rhizosphere against Late Blight Caused by Phytophthora citrophthora on Citrus Fruits  

Kang, So-Young (Major of Plant Resources and Environment, College of Applied Life Sciences, Jeju National University)
Jeun, Yong-Chull (Major of Plant Resources and Environment, College of Applied Life Sciences, Jeju National University)
Publication Information
Research in Plant Disease / v.16, no.1, 2010 , pp. 35-40 More about this Journal
Abstract
Suppression effect of the 12 bacterial isolates from plant rhizosphere against late blight caused by Phytophthora citrophthora were investigated on citrus fruits. Among the bacterial isolates, THJ609-3, TRH423-3, BRH433-2, Lyso-chit and KRY505-3 presented disease suppression after wound inoculation with the fungal pathogen in vivo. The anti-fungal activity was evaluated by measuring the length of inhibition zone of the mycelium P. citrophthora adjacent to the effective bacterial isolates in which all of the 5 bacterial isolates showed antagonistic effects. However, there was no positive correlations between the efficacy of disease suppression and the antagonistic effect. On the other hand, Lyso-chit and KRY505-3 were identified as Bacillus cereus, BRH433-2 as B. circulans and TRH423-3 as Burkholderia gladioli, respectively, by analysis of rDNA sequence on the internal transcript spaces. It is suggested that the effective bacterial isolates may be useful for finding biological control agents against late blight especially on environment-friendly farm where the application of fungicide is limited.
Keywords
Antibiotics; Biological control; Citrus diseases; ITS; Rhizobacteria;
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