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http://dx.doi.org/10.5423/RPD.2009.15.3.209

Physical Characteristics and Antagonistic Effect of Ampelomyces  

Kim, Ji-Young (Plant Medical Research Center, College of Agriculture and Life Science, Chonbuk National University)
Lee, Wang-Hyu (Plant Medical Research Center, College of Agriculture and Life Science, Chonbuk National University)
Kim, Hyung-Moo (Plant Medical Research Center, College of Agriculture and Life Science, Chonbuk National University)
Publication Information
Research in Plant Disease / v.15, no.3, 2009 , pp. 209-216 More about this Journal
Abstract
During the period of June, 2005 to May, 2008, 44 host plants infected with powdery mildew were collected in the Jeon-ju and Jang-su districts of Jeonbuk province and in the Jang-sung district of Jeonnam province. The hyperparasites, Ampelomyces were confirmed in 12 plant species. Most of the pycnidium shapes of Ampelomyces were circular or oval shaped, and the sizes were different even within the same host plant, and also the color of pycnidium was ranged from light brown to dark brown. Ampelomyces species were isolated from 4 hosts including Impatiens balsamina L., Cucurbita pepo, Rudbeckia laciniata var. elatier and Youngia sonchifolia, and thus the most appropriate 12 Ampelomyces strains for the current experiment were selected. When analyzing the selected 12 strains' incubational and nutritional characteristics, the malt extract agar was the most appropriate media. When investigating the effect of osmotic pressure on the spore germination, 0.15M NaCl concentration was the optimum germination concentration. When the isolated Ampelomyces sp. was tested in-vitro, it was found to be effective to control in other plant pathogens, isolated Ampelomyces showed no pathogenicity to the plant. strains isolated . studied on rDNA ITS sequence analysis. The rDNA ITS sequence data of Ampelomyces sp. isolate BSLAH16 from Impatiens balsamina L. were analyzed and identified.
Keywords
Ampelomyces; Hyperparasite; Powdery mildew; Pycnidia fomation; rDNA ITS sequence; Spore germination;
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Times Cited By KSCI : 3  (Citation Analysis)
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