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http://dx.doi.org/10.5423/RPD.2009.15.2.068

Cucumber green mottle mosaic virus Moved into the Non-Host Figleaf Gourd Passing through Cucumber in Grafting System  

Choi, Gug-Seoun (Horticulture & Herbal Environmental Division, National Institute of Horticultural & Herbal Science, RDA)
Lee, Jin-A (Horticulture & Herbal Environmental Division, National Institute of Horticultural & Herbal Science, RDA)
Cho, Jeom-Deog (Horticulture & Herbal Environmental Division, National Institute of Horticultural & Herbal Science, RDA)
Chung, Bong-Nam (Horticulture & Herbal Environmental Division, National Institute of Horticultural & Herbal Science, RDA)
Cho, In-Sook (Horticulture & Herbal Environmental Division, National Institute of Horticultural & Herbal Science, RDA)
Publication Information
Research in Plant Disease / v.15, no.2, 2009 , pp. 68-71 More about this Journal
Abstract
Cucumber green mottle mosaic virus (CGMMV) was not infected in figleaf gourd by sap inoculation. However CGMMV was detected by RT-PCR from the figleaf gourd collected from a field growing cucumber grafted onto figleaf gourd in Cheonan, Chungcheongnam Province in 2008. Which field showed 100% infection rate of the virus disease. In the experiment grafted with cucumber onto figleaf gourd, transportation of CGMMV through cucumber to figleaf gourd was confirmed by RT-PCR when the virus was mechanically inoculated on the leaves of the cucumber. The amplified DNA concentration of the virus on electrophoresis gel was much higher in the cucumber than in the figleaf gourd. However, the virus particles from the figleaf gourds were not observed under electron microscopy, also sap of the figleaf gourds was not transmittable to Nicotiana benthamiana. To identify the existence of CGMMV particle, the virus was purified from figleaf gourd and cucumber growing together in the graft system. CGMMV solution extracted from the cucumber represented a typical absorption spectrum of the virus but that from the figleaf gourd did not. Only a few CGMMV particles were observed in the purified preparation from the figleaf gourd. These results confirmed that CGMMV only passed through figleaf gourd in the grafting system. This study indicated that figleaf gourd is not a host of CGMMY.
Keywords
CGMMV; Cucumber; Figleaf gourd; Graft;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
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