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http://dx.doi.org/10.5423/RPD.2009.15.2.112

Identification and Biological Activity of Two New Phytotoxins Isolated from Botrytis cinerea  

Kim, Geum-Jung (Chemical Biotechnology Research Center, Korea Research Institute of Chemical Technology)
Yoon, Mi-Young (Chemical Biotechnology Research Center, Korea Research Institute of Chemical Technology)
Kim, Heung-Tae (Department of Plant Medicine, Chungbuk National University)
Choi, Gyung-Ja (Chemical Biotechnology Research Center, Korea Research Institute of Chemical Technology)
Jang, Kyoung-Soo (Chemical Biotechnology Research Center, Korea Research Institute of Chemical Technology)
Choi, Yong-Ho (Chemical Biotechnology Research Center, Korea Research Institute of Chemical Technology)
Park, Myung-Soo (Chemical Biotechnology Research Center, Korea Research Institute of Chemical Technology)
Cha, Byeong-Jin (Department of Plant Medicine, Chungbuk National University)
Kim, Jin-Cheol (Chemical Biotechnology Research Center, Korea Research Institute of Chemical Technology)
Publication Information
Research in Plant Disease / v.15, no.2, 2009 , pp. 112-119 More about this Journal
Abstract
We discovered two novel phytotoxins produced by the pathogenic fungus, Botrytis cinerea. Among the twenty-five B. cinerea isolates, which were obtained from various host plants in 1994 and 1996, twenty-two showed strong or moderate pathogenicity on five plants such as cucumber, tomato, red pepper, tobacco and Chinese cabbage. The culture filtrate of the B. cinerea 2-16 strain showed the most potent phytotoxic activity in a tobacco leaf-wounding assay. Two novel phytotoxins were isolated from the liquid cultures of B. cinerea 2-16 by ethyl acetate extraction, flash silica gel column chromatography, silica gel column chromatography, Sephadex LH-20 column chromatography, preparative TLC and subsequently preparative HPLC. Their chemical structures were determined to be 3-O-acetyl botcinol and 3-O-acetyl botcinolide, respectively, by mass and NMR spectral analyses. These two phytotoxins caused leaf necrosis in a leaf-wounding bioassay, and significant electrolyte leakage from leaf tissues of tobacco. In the two bioassays tested, 3-O-acetyl botcinol exhibited stronger phytotoxic activity than 3-O-acetyl botcinolide. This is the first report on the production of both 3-O-acetyl botcinol and 3-O-acetyl botcinolide from B. cinerea.
Keywords
3-O-acetyl botcinol; 3-O-acetyl botcinolide; Botrytis cinerea; Phytotoxin;
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