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http://dx.doi.org/10.5423/RPD.2007.13.1.024

Genetic Diversity of Ralstonia solanacearum Strains Isolated from Pepper and Tomato Plants in Korea  

Seo, Sang-Tae (Horticultural Environment Division, National Horticultural Research Institute, RDA)
Park, Jong-Han (Horticultural Environment Division, National Horticultural Research Institute, RDA)
Han, Kyoung-Suk (Horticultural Environment Division, National Horticultural Research Institute, RDA)
Cheong, Seung-Ryong (Horticultural Environment Division, National Horticultural Research Institute, RDA)
Lee, Seung-Don (Research & Development Bureau, Research Management Division)
Publication Information
Research in Plant Disease / v.13, no.1, 2007 , pp. 24-29 More about this Journal
Abstract
A total of 35 strains of Ralstonia solanacearum isolated from wilted pepper and tomato plants in Korea were analyzed for their genetic diversity by bacteriological, pathological and molecular biological approaches. All the strains were identified as R. solanacearum biovar 4 on the basis of physiological and biochemical tests, and species-specific PCR primers. Pathogenicity of the strains was confirmed by inoculating on 4-week-old pepper and tomato seedlings. Using cluster analysis based on repetitive sequence-based polymerase chain reaction (rep-PCR) genomic fingerprints, R. solanacearum strains isolated from pepper and tomato in Korea divided into 6 groups showing a high degree of genetic diversity at 55% similarity level. The genetic diversify of strains was not significantly correlated with their geographic origins and host plants.
Keywords
Genetic diversity; Pepper; Ralstonia solanacearum; Tomato;
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