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http://dx.doi.org/10.5423/RPD.2006.12.3.254

Selection of Brevibacillus brevis B23 and Bacillus stearothermophilus B42 as Biological Control Agents against Sclerotinia Rot of Lettuce  

Hwang, Ji-Young (Organic Farming Technology Division, National Institute of Agricultural Science and Technology, Rural Development Administration)
Shim, Chang-Ki (Organic Farming Technology Division, National Institute of Agricultural Science and Technology, Rural Development Administration)
Ryu, Kyung-Yeol (Organic Farming Technology Division, National Institute of Agricultural Science and Technology, Rural Development Administration)
Choi, Du-Hoe (Organic Farming Technology Division, National Institute of Agricultural Science and Technology, Rural Development Administration)
Jee, Hyeong-Jin (Organic Farming Technology Division, National Institute of Agricultural Science and Technology, Rural Development Administration)
Publication Information
Research in Plant Disease / v.12, no.3, 2006 , pp. 254-259 More about this Journal
Abstract
Bacillus spp. isolated from mushroom medium wastes were evaluated for their biocontrol potentials on control of Sclerotinia rot of lettuce. The Bacillus isolates were more effectively obtained from waste suspension when directly added into nutrient agar(NA) medium than plating on the agar medium. Totally 42 isolates obtained from the wastes B23 and B42 showed highest antifungal activity against eight fungal pathogens such as Sclerotinia sclerotiorum, Rhizoctonia solani, Pythium ultimum, Phytophthora capsici, Fusarium oxysporum, Colletotrichum gloeosporioides, Cladosporium cucumerinum, and Botrytis cinerea and B23 and B42 were finally selected for further studies. Optimal concentration of the isolates was $10ml(10^7cfu/ml)$ to suppress the Sclerotinia rot of lettuce. Supplements such as starch, glycerol, and egg-yolk successfully maintained the bacterial population for 30 days in vitro and increased bio-control potentials against the disease. The bacterial isolate B23 alone showed 72% control value, furthermore it presented 95% control value when supplemented with 0.2% of starch, glycerol, and egg-yolk. The promising Bacillus isolates B23 and B42 were identified as Brevibacillus brevis and Bacillus stearothermophillus, respectively, based on morphological and physiological characteristics according to API database.
Keywords
Bacillus; Biological control; Lettuce; Mushroom waste; Sclerotinia rot;
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Times Cited By KSCI : 3  (Citation Analysis)
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