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http://dx.doi.org/10.5423/RPD.2006.12.3.226

Characteristics of Cucumber mosaic virus-VCH Causing Vein Chlorosis on Red Pepper in Korea  

Cho, Jeom-Deog (National Horticultural Research Institute, Rural Development Administration)
Lee, Sin-Ho (National Horticultural Research Institute, Rural Development Administration)
Kim, Jeong-Soo (National Horticultural Research Institute, Rural Development Administration)
Choi, Gug-Seoun (National Horticultural Research Institute, Rural Development Administration)
Kim, Hyun-Ran (National Horticultural Research Institute, Rural Development Administration)
Chung, Bong-Nam (National Horticultural Research Institute, Rural Development Administration)
Ryu, Ki-Hyun (Seoul Women's University)
Publication Information
Research in Plant Disease / v.12, no.3, 2006 , pp. 226-230 More about this Journal
Abstract
Cucumber mosaic virus(CMV) was occurred on red pepper showing vein chlorosis or vein necrosis with the incidence rate of 52% from 62 specimens collected in natural fields. Among 32 samples infected with CMV, the specimens of 22 red pepper leaves showing vein chlorosis were infected singly with CMV-VCH. CMV-VCH induced vein chlorosis on the inoculated leaves, and vein banding and vein necrosis on the upper leaves of Nicotiana glutinosa, and then killed after showing bud necrosis. The typical symptoms of vein banding, malformation and blister were produced on the upper leaves of Nicotiana benthamiana and N. tabacum 'Ky-57' without symptoms on the inoculated leaves. The commercial cultivars of 'Bugang', 'Manitta' and 'Gwariput' were shown the typical symptom of vein chlorosis by the mechanical inoculation of CMV-VCH. CMV-VCH was detected specifically by RT-PCR. Virus particles of CMV-VCH were isometric shape having 30 nm diameter. Ultraviolet absorption of purified CMV-VCH was maximum at 260 nm and minimum at 242 nm. The ratio of A260/A280 was 1.71. CMV-VCH had the single nucleo-protein having the molecular weight of 24.5 kDa.
Keywords
Cucumber mosaic virus(CMV-VCH); red pepper; vein chlorosis;
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