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http://dx.doi.org/10.5423/RPD.2004.10.1.058

Contamination Level of Ralstonia solanacearum in Soil of Greenhouses Cultivating tomato Plants in Chungbuk Province and Characteristics of the Isolates  

Yun, Gon-Sig (충북대학교 식물의학과)
Park, Sang-Yong (충북대학교 식물의학과)
Kang, Hyo-Jung (충북농업기술원)
Lee, Ki-Yeol (충북농업기술원)
Cha, Jae-Soon (충북대학교 식물의학과)
Publication Information
Research in Plant Disease / v.10, no.1, 2004 , pp. 58-62 More about this Journal
Abstract
Contamination level and characteristics of Ralstonia solanacearum in soil of greenhouses cultivating tomato plants in Chungbuk province was determined. R. solanacearum was isolated with the semiselective media in 27 greenhouse soil samples out of 133 greenhouses soil investigated, which indicates 20.3 ％ of tomato cultivating greenhouses in Chungbuk contaminated with the bacterial wilt pathogen. Density of R. solanacearum was estimated to 10$^{2.4}$ cfu/g in the contaminated soil. All 71 isolates of R. solanacearum which containing 12 isolates from the diseased tomato plants were race 1, ann 35 isolates of them were biovar 3 and 36 isolates were biovar 4. More than 88％ of 71 isolates were inhibited growth on nutrient agar containing oxolinic acid 0.5 $\mu\textrm{g}$/ml, streptomycin 25 $\mu\textrm{g}$/ml, tetracycline 5 $\mu\textrm{g}$/ml and cupric sulfate 375 $\mu\textrm{g}$/ml (1.5 mM). The 11.3％, 4.2％ and 5.6 ％ of the isolates can grow on nutrient agar containing 10 times more oxolinic acid, streptomycin, tetracycline than minimal inhibitory concentration of the sensitive strains. Five isolates were resistant to 2 bactericides and one isolates was resistant to all 3 bactericides.
Keywords
Bactericide resistance; Biovar; Contamination level; Race; Ralstonia solanacearum; Tomato plants;
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1 Buddenhagen, I. and Kelman, A. 1964. Biological and physiological aspects of bacterial wilt caused by Pseudomonas solanacearum. Annu. Rev Phytopathol. 2: 203-230   DOI   ScienceOn
2 Denny, T. P and Hayward, A. C. 2001.II Gram-negative bacteria. Ralstonia. In Laboratory guide for identification of plant pathogenic bacteria. pp151-174. ed. by N. W. Schaad, J. B. Jones, and W. Chun, The American Phytopathological Society Press
3 호국식물보호학회. 1998. 한국식물병목록. 144pp
4 Hayward, A. C. 1991. Biology and epidemiology of bacterial wilt caused by Pseudomonas solanacearum. Annu. Rev. Phytopathol. 29: 65-87   DOI   PUBMED   ScienceOn
5 Miller, L. T 1982. Single derivatization method for routine analysis of bacterial whole-cell fatty acid methyl esters, including hydroxyl acids. J. Clin. Microbiol. 16: 584-586
6 Weller, S. A., Elphinstone, J. G., Smith, N. c, Boonham, N. and Stead, D. E. 2000. Detection of Ralstonia solanacearum strains with a quantitative, multiplex, real-time, fluorogenic PCR (TaqMan) assay. Appl. Environ. Microbiol. 66: 2853-2858   DOI   ScienceOn
7 농림부. 2002. 농림통계연보. 107pp
8 농업과학기술원 작물보호부 2002. 감자 풋마름병 방제기술 개발 농촌진흥청
9 이영근, 김정화, 강여규. 1982. 우리나라 담배 세균성마름병(立枯病菌: Pseudomonas solanacearum)의 race와 biochemical type. 한국식물보호학회지 21: 123-127
10 Griep, R. A., van Twisk, C., van Beckhoven, J. R. C. M., van der Wolf, J. M. and Schots, A. 1998. Development of specific recombinant monoclonal antibodies against the lipopolysccharide of Ralstonia solanacearum race 3. Phytopathol. 88: 795-803   DOI   ScienceOn
11 Ito, S., Ushijima, Y, Fujii, T, Tanaka, S., Kameya-Iwski, M., Yoshiware, S. and Kishi, F. 1998. Detection of viable cells of Ralstonia solanacearum in soil using a serniselective medium and a PCR technique. Phytopathol. 146: 379-384   DOI   ScienceOn
12 이승돈. 1999. 한국의 주요 식물세균병 발생 및 특성. 서울대학교 농학박사학위논문