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http://dx.doi.org/10.5423/RPD.2003.9.3.107

Chinese yam necrotic mosaic virus Isolated from Chinese Yam in Korea  

Kang, Dong-Kyoon (Experiment Station of Bioresoures, Gyeungbuk Provincial ATA)
Kondo, Toru (Aomori Green Biocenter)
Shin, Jong-Hee (Experiment Station of Bioresoures, Gyeungbuk Provincial ATA)
Shin, Hye-Young (Dept. Biology, Yeungnam University)
Sung, Jung-Hyun (Dept. Biology, Yeungnam University)
Kang, Sang-Gu (Institute of Biotechnology, Yeunganam University)
Chang, Moo-Ung (Dept. Biology, Yeungnam University)
Publication Information
Research in Plant Disease / v.9, no.3, 2003 , pp. 107-115 More about this Journal
Abstract
Chinese yam (Dioscorea opposita cv. Jang-Ma) plants showing necrotic mosaic symptom were collected from their growing fields in Andong, Euisong, Gunwi and Daegu, Korea. Direct negative stainning method by electron microscope showed filamentous particles of about 660 nm in length. Immunosorbent electron microscopy (ISEM) revealed filamentous particles of 660nm decorated with antiserum of Chinese yam necrotic mosaic virus (ChYNMV). The virues purified partially were used to isolate viral RNA as template for RT-PCR to amplify about 1.2 kbp of 3'-terminal region (coat protein, 3'-UTR) with ChYNMV specific and oligo-dT primers. Amino acids sequences of amplified CP genes revealed that the viruses shared 97.9% similarity with ChYNMV (AB044386) wh ich was known as the member of Macluravirus. So the viruses from Chinese yam (D. opposita cv. Jang-Ma) plants were identified as ChYNMV. Comparing the CP amion acid sequences of ChYNMV strains with other macluraviruses such as Cardamon mosaic virus (CdMV), Narcissus latent virus (NLV) and Maclura mosaic virus (MacMV) revealed that N-terminal was the most varialbe region and conserved regions were present within the genus Macluravirus.
Keywords
Chinses yam necrotic mosaic virus; coat protein gene; Dioscorea opposita; Macluravirus;
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