Browse > Article
http://dx.doi.org/10.11110/kjpt.2015.45.1.36

Melia toosendan and M. azadarach are a single species due to their genetic similarity  

Kim, Hoe-Won (Division of Life Sciences, School of Life Sciences, Korea University)
Yeon, Seung-Woo (ILDONG Research Laboratories, ILDONG Pharmaceutical Co. Ltd.)
Kim, Ki-Joong (Division of Life Sciences, School of Life Sciences, Korea University)
Publication Information
Korean Journal of Plant Taxonomy / v.45, no.1, 2015 , pp. 36-44 More about this Journal
Abstract
The nucleotide sequences of six markers, including nuclear ITS, chloroplast matK, rbcL, atpF-H, psbK-I and psbA-trnH, were analyzed for the plants known as Melia toosendan collected in Southwest China; M. azadarach planted in Southeast China, Korea and India; and species related to Sapindaceae in order to clarify the species boundary between M. toosendan and M. azadarach. The result of a phylogenetic analysis using the nuclear ITS and five chloroplast marker sequences determined that the plants known as M. toosendan and M. azadarach are the same species. These two species have been treated as a single species or as two different species depending on the researcher. The result of the present study supports the contention that the two species are the same. In addition, a sister species to M. azadarach registered in various countries with various basionyms is Azadirachta indica, a well-known medicinal plant. It has previously been classified as a member of the genus Melia.
Keywords
Melia toosendan; Melia azadarach; Phylogenetic analysis;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 Mabberley, D. J., C. M. Pannell and A. M. Sing. 1995. Meliaceae. Flora Malesiana Series I Spermatophyta. Vol. 12. Paperback. Pp. 1-407.
2 Moon, B. C., W. J. Kim, Y. Ji, Y. M. Lee, Y. M. Kang, G. Choi and H. K. Kim. 2014. Development of Molecular Marker for the authentication of Patriniae Radix by the analysis of DNA barcodes. The Korea Journal of Herbology 29(6): 45-53.   DOI
3 Muellner, A. N., R. Samuel, M. W. Chase, A. Coleman and T. F. Stuessy. 2008. An evaluation of tribes and generic relationships in Melioideae (Meliaceae) based on nuclear ITS ribosomal DNA. Taxon 57: 98-108.
4 Muellner, A. N., Samuel., R., Johnson, S. A., Cheek, M., Pennington, T. D. and Chase, M. W. 2003. Molecular phylogenetics of Meliaceae (Sapindales) based on nuclear and plastid DNA sequences. American Journal of Botany 90: 471-480.   DOI
5 Peng, H. and D. J. Mabberley. 2008. In Flora of China. Vol. 11. Wu, Z. Y., P. H. Raven and D. Y. Hong (eds.), Science Press, Beijing, and Missouri Botanical Garden Press, St. Louis. Pp. 130-131.
6 Pennington, T. D., Styles B. T. 1975. A generic monograph of the Meliaceae. Blumea 22: 419-540.
7 Pennington, T. D., B. T. Styles and D. A. H. Taylor. 1981. Meliaceae. Flora Neotropica Monographs 28. The New York Botanical Garden, Bronx.
8 Posada, A. D. and K. A. Crandall. 1998. Modeltest: Testing the model of DNA substitution. Bioinformatics 14: 817-818.   DOI
9 Siebold, P. F and J. G. Zuccarini. 1843. Abhandlungen der Mathematisch-Physikalischen Classe der Kniglich Bayerischen Akademie der Wissenschaften 4(2): 159.
10 CBOL Plant Working Group. 2009. A DNA barcode for land plants. Proceedings of the National Academy of Sciences USA 106: 12794-12797.   DOI
11 Chinese Pharmacopoeia Commission. 2010. Pharmacopoeia of the People's Republic of China. China Medical Science Press, Beijing
12 Doyle, J. J. and J. L. Doyle. 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin 19: 11-15.
13 Drummond, A. J., B. Ashton, S. Buxton, M. Cheung, A. Cooper, C. Duran, M. Field, J. Heled, M. Kearse, S. Markowitz, R. Moir, S. Stones-Havas, S. Sturrock, T. Thierer and A. Wilson. 2011. Geneious v5.4. from http://www.geneious.com
14 Edgar, R. C. 2004. MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Research 32: 1792-1797.   DOI
15 Kim, J. H. 2007. Meliaceae. In The Genera of Vascular Plants of Korea. Park, C.-W. (ed.), Academy Publishing Co., Seoul. Pp. 707-708.
16 Farris, J. S., V. A. Albert, M. Kllersj, D. Lipscomb and A. G. Kluge. 1996. Parsimony jackknifing outperforms neighborjoining. Cladistics 12: 99-124.   DOI
17 Felsenstein, J. 1985. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39: 783-791.   DOI
18 Kim, D. K. 2001. Pharmacognosy, Dongmyung publishing Co., Seoul, Pp. 132-133. (in Korean)
19 Kim, K. J. and R. K. Jansen. 1994. Comparisons of phylogenetic hypotheses among different data sets in dwarf dandelions (Krigia, Asteraceae): additional information from internal transcribe spacer sequences of nuclear ribosomal DNA. Plant Systematics and Evolution 190: 157-185.   DOI
20 Kim, K. J. 1996. Combination of different data sets for phylogeny reconstruction. Korean Journal of Plant Taxonomy 26: 293-327. (in Korean)
21 Kim, R. S. 1998. Meliaceae. In Flora Coreana. Vol. 4. Im, R. J. (ed.), Science and Technology Publishing House, Pyongyang. Pp. 204-207. (in Korean)
22 Kimura, M. 1980. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution 16: 111-120.   DOI
23 Korea Food and Drug Administration. 2012. The Korean Herbal Pharmacopoeia. The Korea Food and Drug Adminstration Notification No. 2012-135. Pp. from http://www.law.go.kr/LSW/admRulLsInfoP.do?chrClsCd=&admRulSeq=2000000021929
24 Lee, Y. N. 2006. New Flora of Korea. Vol. I. Kyo-Hak publishing Co., Seoul. Pp. 658-686. (in Korean)
25 White, T. J., T. Bruns, S. Lee and J. W. Taylor. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In PCR Protocols: A Guide to Methods and applications. Innis, M. A., D. H. Gelfand, J. J. Sninsky and T. J. White. (eds.). Academic Press, Inc., New York. Pp. 315-322.
26 Son, O G., B. I. Seo, S. H. Lee and S. J. Park. 2014. The Study of DNA markers to identify of Allium sativum L.. The Korea Journal of Herbology 29: 27-33.
27 Swofford, D. L. 2002. PAUP: Phylogenetic Analysis Using Parsimony and other Methods (ver. 4.0), Sunderland, MA Sinauer Associates.