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Analysis of ITS DNA Sequences of Korean Oxalis Species (Oxalidaceae)  

Koo, Jachoon (Division of Science Education, Chonbuk National University)
Chae, Mi Suk (Division of Science Education, Chonbuk National University)
Lee, Jeoung-Ki (Division of Science Education, Chonbuk National University)
Whang, Sung Soo (Division of Science Education, Chonbuk National University)
Publication Information
Korean Journal of Plant Taxonomy / v.37, no.4, 2007 , pp. 419-430 More about this Journal
Abstract
This study was conducted to know the taxonomic features of nuclear ribosomal ITS DNA sequences, ITS1, ITS3 and 5.8S regions, as to nine individuals belonging to four Oxalis species in Korea and an induced species. Sequences of the same regions of sixteen taxa deposited in GenBank were also aligned with those of Korean species as outgroups. The length of ITS sequences aligned in this study is 679 by in total. Evidences, from not only the sequence similarities and divergences but also the phylogenetic and statistical treatments with ITS sequences aligned, were useful for the taxonomy of the genus. The similarity of sequences, among both cauline and acauline taxa, is high as 89% and 95% respectively, but between cauline and acauline taxa, relatively low in the range of 64~69%. The sequence divergences, among both cauline and acauline taxa, is also high as much as 0.36~0.42, but between both cauline and both acauline taxa, low as 0.04~0.06. Two groups between cauline and acauline taxa are paraphyletic, and each group makes a single Glade with a high bootstrap value. The analysis of variance, using ITS sequence aligned, revealed that taxa are significantly different in the level of 0.5%, and O. corymbosa, an induced speices, is also separated from the Korean taxa in the Duncan analysis.
Keywords
ANOVA; ITS sequences; Oxalis; phylogeny;
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