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Genetic Diversity in Three Populations of Hibiscus hamabo(Malvaceae) in Jeju Island, Korea  

Kim, Young-Dong (Department of Life Science, Hallym University)
Kim, Ki-Joong (School of Life Sciences & Biotechnology, Korea University)
Kim, Sung-Hee (Department of Life Science, Hallym University)
Kim, Hyeong-Tae (School of Life Sciences & Biotechnology, Korea University)
Publication Information
Korean Journal of Plant Taxonomy / v.37, no.2, 2007 , pp. 115-129 More about this Journal
Abstract
Using internal transcribed spacer (ITS) sequences and inter-simple sequence repeats (ISSRs) data, genetic diversity of a rare species, Hibiscus hamabo Siebold & Zucc. was examined for 3 populations in Jeju Island, Korea. A total of 14 nucleotide (excluding 3 ambiguous nucleotide) site variation in the ITS was observed from 18 individuals (Population 1, Hadori), which differed up to 13 bp in pair-wise comparison. On the contrary, the ITS sequences of all individuals in Populations 2 and 3 were identical. Genetic diversity estimates including Nei's gene diversity (h) generated by ISSR data were substantially high in Population 1 compared to other two populations. Low genetic variation in Populations 1 and 2 is considered due to genetic drift (bottleneck effect) and limited gene flow in these populations. Considering the differences in genetic diversity, protection of the Population 1(Hadori) is very critical for in situ conservation of Hibiscus hamabo in Korea. If ex situ conservation is required, making the full use of Population 1 will be most efficient.
Keywords
Conservation; Hibiscus hamabo; ISSR; ITS; rare plant;
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1 Schaal, B. A, D. A. Hayworth, K. M. Olsen, J. T. Rauscher and W. A. Smith. 1998. Phylogeographic studies in plants: problems and prospects. Molecular Ecology 7:465-474   DOI   ScienceOn
2 Andreasen, K. and B. Baldwin. 2001. Unequal evolutionary rates between annual and prerenial lineages of checker mallows (Sidalcea, Malvaceae): Evidence from 18S-26S rDNA internal and external transcribed spacers. Mol. Biol. Evol. 18:936-944   DOI   ScienceOn
3 Camacho, F. J and A. Liston. 2001. Population structure and genetic diversity of Botrychium pumicola (Ophioglossaceae) based on inter-simple sequence repeats (ISSR). Amer. J. Bot. 88: 1065-1070   DOI   ScienceOn
4 Kim, Y.-D., C.-W. Park, B.-Y. Sun, K.- J. Kim, E.- J. Lee and S.- H. Kim. 2005. ITS sequence variations in common ragweed and giant ragweed. Korean J. PI.Taxon. 35: 273-285 (in Korean)   DOI
5 Kimura, M. 1980. A simple method for estimating evolutionary rate of the base substitution through comparative studies of nucleotide sequences.. J. Mol. Evol. 16: 111-120   DOI
6 Milligan, B. G., J. Leebens-Mack and A. E. Strand. 1994. Conservation genetics: beyond the maintenance of marker diversity. Molecular Ecology 12: 844-855
7 Palmer, J. D. 1986. Isolation and structural analysis of chloroplast DNA Meth. Enzymol. 118: 167-186   DOI
8 Soltis P. S. and R. K. Kuzoff. 1993. ITS sequence variation within and among populations of Lomatium grayi and L. laevigatum (Umbelliferae). Mol. Phylogenet. Evol. 2:166-170   DOI   ScienceOn
9 Thompson, J. D., D. G. Higgins and T. J. Gibson. 1995. CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positions-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22: 4673-4680   DOI   ScienceOn
10 White, T. J., T. Birns, S. Lee and J. Taylor. 1990. Amplification and Direct Sequencing of Fungal Ribosomal RNA Genes for Phylogenetics. In PCR Protocols: A Guide to Methods and Applications, Innis, M., D. Gelfand, J. Sninsky and T. White (eds.), Academic Press, San Diego, Pp, 315-322
11 Nei, M. 1973. Analysis of gene diversity in subdivided population. Proc. Nat. Acad. Sci. USA 70: 3321-3323
12 Xiao, M., Q. Li, L. Wang, L. Guo, J. Li, L. Tang and F. Chen. 2006. ISSR analysis of the genetic diversity of the endangered species Sinopodophyllum hexandrum (Royle) Ying from Western Sichuan Province, China. J. Integrative Plant Biology 48:1140-1146   DOI   ScienceOn
13 Doyle, J. J. and J. L. Doyle. 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem. Bull. 19: 11-15
14 Avise, J C. 1994. Molecular markers, natural history and evolution. Chapman and Hall, London
15 Hamrick J. L. and M. J. W. Godt. 1996. Conservation Genetics of Endemic Plant Species. In: Avise J. C; Hamrick J. L., eds. Conservation Genetics: Case Histories from Nature. New York: Chapman and Hall, pp 281-304
16 Swofford, D. L. 2002. PAUP*, phylogenetic analysis using parsimony (*and other methods), version 4.0b. Sinauer, Sunderland
17 Ge, xj, Y. Yu, Y.-M. Yuan, H.-W. Huang and C. Yan. 2005. Genetic diversity and geographic differentiation in endangered Ammopiptanihus (Leguminosae) populations in desert regions of northwest China as revealed by ISSR analysis. Ann. Bot. 95: 843-851   DOI   ScienceOn
18 Chung, J. M., B. C. Lee, J. S. Kim, C.-W. Park, M. Y Chung and M. G. Chung. 2006. Fine-scale genetic structure among genetic individuals of the clone-forming monotypic genus Echinosophora koreensis (Fabaceae). Annals of Botany 98:165-173   DOI   ScienceOn
19 Jousson, O., J. Pawlowski, L. Zaninetti, F. W. Zechman, F. Dini, G. Di Guiseppe, R. Woodfield, A. Millar and A. Meinesz. 2000. Invasive alga reaches California. Nature 408: 157-158   DOI
20 Yeh, F. C. R. C. Yang and T. Boyle. 1999. POPGENE. Microsoft Windows-based Freeware for Population Genetic Analysis. Release 1.31. Edmonton: University of Alberta, Canada
21 Xiao, L.-Q., X.-J. Ge, X. Gong, G. Hao and S.-X. Zheng. 2004. ISSR variation in the endemic and endangered plant Cycas guizhouensis (Cycadaceae). Ann. Bot. 94:133-138   DOI   ScienceOn
22 Kim, Y.-D., S.-H. Kim and L. R. Landrum. 2004. Taxonomy and phytogeographic implications from ITS phylogeny in Berberis (Berberidaceae), J. Plant Res. 117:175-182